Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)

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Flow Cytometry - Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)

    Key features and details

    • Mouse monoclonal [GR-3G7] to Clostridium botulinum Toxin B
    • Suitable for: Flow Cyt, Competitive ELISA
    • Isotype: IgG1

    Overview

    • Product name

      Anti-Clostridium botulinum Toxin B antibody [GR-3G7]

    • Description

      Mouse monoclonal [GR-3G7] to Clostridium botulinum Toxin B

    • Host species

      Mouse

    • Specificity

      This antibody reacts with Clostridium botulinum Toxin B.

    • Tested applications

      Suitable for: Flow Cyt, Competitive ELISAmore details

    • Species reactivity

      Reacts with: Other species

    • Immunogen

      Genetic immunisation with cDNA encoding Clostridium botulinum Toxin B.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.20
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Protein G purified

    • Clonality

      Monoclonal

    • Clone number

      GR-3G7

    • Isotype

      IgG1

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab59715 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 1.2µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    Competitive ELISA 1/200 - 1/400.

    Target

    • Relevance

      Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the 76 Gln | Phe 77 bond of synaptobrevin 2.

    • Cellular localization

      Secreted

    • Alternative names

      • BoNT/B antibody
      • Bontoxilysin B antibody
      • BontoxilysinB antibody
      • bot B antibody
      • botB antibody
      • Botulinum neurotoxin type B antibody
      see all

    Images

    • Flow Cytometry - Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)
      Flow Cytometry - Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)
      BOSC23 cells were transiently trans-fected with an expression vector encoding either Clostridium botulinum Toxin B (red curve) or an irrelevant protein (control transfectant). Binding of ab59715 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with Clostridium botulinum Toxin B transfected cells.

    References (0)

    Publishing research using ab59715? Please let us know so that we can cite the reference in this datasheet.

    ab59715 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Hello, I just wanted to be check that this antibody binds to the whole toxin protein, and not just the light or heavy chain. Please let me know. Thank you,

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    Answer

    Thank you for contacting us with your question and for your patience while I have been in touch with the lab about ab59715. This antibody was raised against the light chain and has only been tested for reactivity with the light chain, so it most likely does not recognize the heavy chain. The rabbit polyclonal ab20899 might react with both the heavy and light chains, but I will need to check on that with the lab and I'll get back to you. Please let me know if you have any questions, and I'll let you know once I hear back about ab20899.

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