Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)

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Overview

  • Product name

    Anti-Clostridium botulinum Toxin B antibody [GR-3G7]

  • Description

    Mouse monoclonal [GR-3G7] to Clostridium botulinum Toxin B

  • Host species

    Mouse

  • Specificity

    This antibody reacts with Clostridium botulinum Toxin B.

  • Tested applications

    Suitable for: Flow Cyt, Competitive ELISAmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Genetic immunisation with cDNA encoding Clostridium botulinum Toxin B.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    GR-3G7

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1.2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
Competitive ELISA 1/200 - 1/400.

Target

  • Relevance

    Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the 76 Gln | Phe 77 bond of synaptobrevin 2.

  • Cellular localization

    Secreted

  • Alternative names

    • BoNT/B antibody
    • Bontoxilysin B antibody
    • BontoxilysinB antibody
    • bot B antibody
    • botB antibody
    • Botulinum neurotoxin type B antibody
    see all

Images

  • Flow Cytometry - Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)
    Flow Cytometry - Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)
    BOSC23 cells were transiently trans-fected with an expression vector encoding either Clostridium botulinum Toxin B (red curve) or an irrelevant protein (control transfectant). Binding of ab59715 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with Clostridium botulinum Toxin B transfected cells.

References

ab59715 has not yet been referenced specifically in any publications.

Publishing research using ab59715? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Hello, I just wanted to be check that this antibody binds to the whole toxin protein, and not just the light or heavy chain. Please let me know. Thank you,

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Answer

Thank you for contacting us with your question and for your patience while I have been in touch with the lab about ab59715. This antibody was raised against the light chain and has only been tested for reactivity with the light chain, so it most likely does not recognize the heavy chain. The rabbit polyclonal ab20899 might react with both the heavy and light chains, but I will need to check on that with the lab and I'll get back to you. Please let me know if you have any questions, and I'll let you know once I hear back about ab20899.

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