Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)
![Flow Cytometry - Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)](https://www.abcam.com/ps/products/59/ab59715/Images/ab59715_1.jpg "Flow Cytometry - Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)")
Key features and details
- Mouse monoclonal [GR-3G7] to Clostridium botulinum Toxin B
- Suitable for: Flow Cyt, Competitive ELISA
- Isotype: IgG1
Overview
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Product name
Anti-Clostridium botulinum Toxin B antibody [GR-3G7] -
Description
Mouse monoclonal [GR-3G7] to Clostridium botulinum Toxin B -
Host species
Mouse -
Specificity
This antibody reacts with Clostridium botulinum Toxin B. -
Tested applications
Suitable for: Flow Cyt, Competitive ELISAmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Genetic immunisation with cDNA encoding Clostridium botulinum Toxin B.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
GR-3G7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1.2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Competitive ELISA |
1/200 - 1/400.
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| Notes |
|---|
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Flow Cyt
Use 1.2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Competitive ELISA
1/200 - 1/400. |
Target
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Relevance
Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the 76 Gln | Phe 77 bond of synaptobrevin 2. -
Cellular localization
Secreted -
Alternative names
- BoNT/B antibody
- Bontoxilysin B antibody
- BontoxilysinB antibody
see all
Images
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Flow Cytometry - Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)BOSC23 cells were transiently trans-fected with an expression vector encoding either Clostridium botulinum Toxin B (red curve) or an irrelevant protein (control transfectant). Binding of ab59715 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with Clostridium botulinum Toxin B transfected cells.
Datasheets and documents
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Datasheet download
References (0)
ab59715 has not yet been referenced specifically in any publications.
