Anti-Clostridium difficile Toxin A and B antibody (ab211057)

Overview

  • Product name

    Anti-Clostridium difficile Toxin A and B antibody
  • Description

    Chicken polyclonal to Clostridium difficile Toxin A and B
  • Host species

    Chicken
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Clostridium difficile
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Clostridium difficile Toxin A and B aa 739-751. Toxin B (Clostridium difficile)
    Sequence:

    ANQYEVRINSEGR


    Database link: P18177

    Synthetic peptide corresponding to Clostridium difficile Toxin A and B aa 741-753. Toxin A (Clostridium difficile)
    Sequence:

    ANQYEVRINSEGR


    Database link: P16154

  • Positive control

    • Clostridium difficile culture supernatants.
  • General notes

    For long term storage: Aliquot, add 10-50% glycerol and store at -20°C.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgY

Associated products

Applications

Our Abpromise guarantee covers the use of ab211057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 270, 308 kDa.

Target

  • Relevance

    Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.
  • Database links

    • Alternative names

      • C. difficile Toxin B antibody
      • Clostridium difficile Toxin B antibody
      • Cytotoxin B antibody
      • tcdA antibody
      • tcdB antibody
      • toxA antibody
      • toxB antibody
      • Toxin B antibody
      see all

    Images

    • All lanes : Anti-Clostridium difficile Toxin A and B antibody (ab211057) at 1/1000 dilution

      Lane 1 : Purified Toxin A from Clostridium difficile culture supernatant
      Lane 2 : Clostridium difficile VP1 (toxigenic strain) culture supernatant
      Lane 3 : Clostridium difficile R20291 (moderate toxigenic strain) culture supernatant
      Lane 4 : Clostridium difficile 318 (non-toxigenic strain) culture supernatant

      Lysates/proteins at 30 µl per lane.

      Secondary
      All lanes : Alpaca Aurora-HRP at 1/5000 dilution

      Predicted band size: 270, 308 kDa

    References

    This product has been referenced in:

    • Stone NE  et al. Domestic canines do not display evidence of gut microbial dysbiosis in the presence of Clostridioides (Clostridium) difficile, despite cellular susceptibility to its toxins. Anaerobe N/A:N/A (2019). Read more (PubMed: 30946985) »
    See 1 Publication for this product

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