Overview

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.
  • Cellular localization

    Secreted
  • Database links

    • Alternative names

      • tcdA antibody
      • toxA antibody

    References

    ab93318 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for contacting us in regards to the anti-Clostridium difficile Toxin A antibody ab35021 and ab93318.

    As discussed over the phone, as both ab35021 and ab93318 are rabbit polyclonal antibodies and are raised against the same peptide sequence, I would not suggest using these as an ELISA pair.

    Using an antibody such as https://www.abcam.com/Clostridium-difficile-Toxin-A-antibody-PCG4-ab19953.htmlwould prove more advantageous as it is raised in a different species (and can therefore be specifically differentiated from the other antibodyby the secondary antibody used). It has been raised against the full length Toxin A protein and is likely (but not guaranteed)to detect a different epitope to ab35021 and ab93318.

    If you would like to try ab35021or ab93318 with ab19953 in a sandwich ELISA, I can offer you a testing discount.This would involve you purchasingab19953, testing it in a sandwich ELISA and sharing the results with us through submitting an Abreview. No matter whether the results are positive or negative you would be eligible for a free primary antibody of your choice from our catalog. More information on this offer can be found here:

    https://www.abcam.com/collaboratordiscount

    The only constraint in the offer is that the antibody to be tested needs to be purchased, the antibody tried, the Abreview submitted and the "free" antibody claimed within 4 months. If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase ofab19953.

    In terms of the Abreview, we would not need explicit details of the experiment carried out, just enough to know how well the antibodies performed, so a graph showing binding with increasing sample, and some protocol details such as the antibody concentrations andblocking conditions used.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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