Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23359-15] to Clostridium difficile Toxin A - BSA and Azide free
- Suitable for: WB
Product nameAnti-Clostridium difficile Toxin A antibody [EPR23359-15] - BSA and Azide free
See all Clostridium difficile Toxin A primary antibodies
DescriptionRabbit monoclonal [EPR23359-15] to Clostridium difficile Toxin A - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Predicted to work with: Clostridium difficile
Recombinant fragment within Clostridium difficile Toxin A aa 1-550. The exact sequence is proprietary. Clostridioides difficile
Database link: P16154
- WB: Native Clostridium difficile Toxin A protein.
ab273507 is the carrier-free version of ab272720. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab273507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 308 kDa (predicted molecular weight: 308 kDa).|
RelevanceClostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.
- SwissProt: P16154 Clostridium difficile
- tcdA antibody
- toxA antibody
All lanes : Anti-Clostridium difficile Toxin A antibody [EPR23359-15] (ab272720) at 1/1000 dilution
Lane 1 : Native Clostridium difficile Toxin A protein (ab123999) 10ng
Lane 2 : Native Clostridium difficile Toxin B protein (ab124001) 10ng
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 308 kDa
Observed band size: 308 kDa
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Both native proteins were from Abcam.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272720).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab273507 has not yet been referenced specifically in any publications.