Overview

  • Product name
    Anti-Clostridium difficile Toxin A antibody [PCG4]
    See all Clostridium difficile Toxin A primary antibodies
  • Description
    Mouse monoclonal [PCG4] to Clostridium difficile Toxin A
  • Host species
    Mouse
  • Specificity
    C. difficile Toxin A. Does not cross react with C. difficile Toxin B
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Clostridium difficile
  • Immunogen

    Full length protein (C. difficile).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    >98% pure (SDS-PAGE). Protein G chromatography.
  • Clonality
    Monoclonal
  • Clone number
    PCG4
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 308 kDa. PubMed: 24244530
ICC/IF Use at an assay dependent concentration. PubMed: 21266163
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.
  • Cellular localization
    Secreted
  • Database links
    • Alternative names
      • tcdA antibody
      • toxA antibody

    References

    This product has been referenced in:
    • Darkoh C  et al. Accessory Gene Regulator-1 Locus Is Essential for Virulence and Pathogenesis of Clostridium difficile. MBio 7:N/A (2016). WB . Read more (PubMed: 27531912) »
    • Dhillon HS  et al. Homogeneous and digital proximity ligation assays for the detection of Clostridium difficile toxins A and B. Biomol Detect Quantif 10:2-8 (2016). Read more (PubMed: 27990343) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Application
    Flow Cytometry
    Sample
    Bacteria Cell (Human fecal bacteria)
    Permeabilization
    No
    Gating Strategy
    Bacterias which binding to C.diff toxin A
    Specification
    Human fecal bacteria
    Preparation
    Cell harvesting/tissue preparation method: Cell harvesting/tissue preparation method: Approximately 50mg of formaldehyde-fixed fecal material was homogenized in 10ml of sterile,non-bacteriostatic PBS.The slurry was then passed through 0.25mm stainless steel laboratory sieve for 3 times.The resulting material was centrifuged at 6000*g for 15min.The resulting concentrated fecal bacteria was resuspended to a final concentration of 1*10^8 CFU/ml.
    Sample buffer: Phosphate Buffer solution
    Fixation
    Formaldehyde

    Qianyun Lin

    Verified customer

    Submitted Nov 07 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Bacteria Purified protein (Purified toxin A protein from cultures supernatant)
    Gel Running Conditions
    Non-reduced Denaturing (NuPAGE 3-8% Tris-Acetate Gel)
    Loading amount
    10 µg
    Specification
    Purified toxin A protein from cultures supernatant
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Qianyun Lin

    Verified customer

    Submitted Nov 06 2018

    Answer

    I can confirm that the starting material of this product was ascites. Each lot of ascites is tested against C. difficile Toxin A (purified from bacterial broth growth) coated on the microtiter plate. Ascites was tested as a dilution series followed by goat anti-mouse:HRP as the secondary antibody conjugate.

    Read More

    Answer

    Thank you for your enquiry and your interest in our products.
    I can confirm that this antibody (ab19953:Anti-Clostridium difficile Toxin A antibody [PCG4]) was launched on June 2006.
    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Answer

    Thank you for letting me know this. I have now updated the species list.

    If you would like to submit similar reviews for the other antibodies you have used please do. Each new review will earn you more Abpoints which can be used to claim money off future Abcam orders or Amazon vouchers. Please refer tothe following linkfor further information as to how the Abpoints can be used:

    https://www.abcam.com/index.html?pageconfig=loyalty_about

    I look forward to receiving your review.

    Read More

    Answer

    I have now returned from the long weekend, I hope the delay in my reply has not affected your work.

    Thank you for providing me with this feedback of how you found the anti-C. difficile toxin antibodies to work. In regards to the anti-Clostridium difficile Toxin A antibody [B618M] (ab82285), strictly speaking this antibody would not be covered by the Abpromise for the experiment you have been performing as we have not tested it in ELISA ourselves and it is not listed as an application on the datasheet of the antibody. As you say, I would expect the antibody to work in ELISA but due to the potential difference in how the epitope is presented in the different sample types the antibody may not be suitable for it.

    I understand the effort you have put into optimising these antibodies and I would be willing to bend the rules slightly for you. I canoffer youareplacementantibody of your choice from our catalogue if you would be willing to provide an Abreview for ab82285 with ELISA. Information such as that you have gathered is very useful for future customers in choosing the antibodies suitable for them. This Abreview should only take 5-10 minutes to complete, more information on the process can be found here:

    https://www.abcam.com/abreviews

    Of course if you would be willing to provide Abreviews for the other antibodies you have been using this would be very useful. As a reward for providing Abreviews for any of our products you receive Abpoints. These can be collected and used against the cost of future orders or claimed as Amazon vouchers. More information on the Abpoints system can be found from the following link:

    https://www.abcam.com/abpoints

    If you would be willing to provide the Abreview for ab82285 please do let me know and I will arrange for a discount code to be generated for you to claim the replacement antibody.

    Thank you for raising your concerns in regards to the concentration of ab77583. I am currently seeking further information from the source of this antibody to confirm exactly what the concentration of this antibody was determined to be. Once I have this information I will get back to you.

    I look forward to receiving your reply.

    Read More

    Answer

    Vielen Dank für Ihren Anruf.

    Zu den Toxinen kann ich Ihnen folgende Informationen aus dem Labor weiterleiten:

    Clostridium difficile Toxin A Protein ab123999und Clostridium difficile Toxin B Protein ab124001werden aus demClostridium difficile Stamm VPI10463 aufgereinigt, der Toxinotyp 0-Toxine produziert.

    Beide Toxine sind aktiv; jeder Batch wird im Verocytotoxicity Assay getestet und ist toxisch für Zellen bis zu einer Konzentration von mindestens5 ng/ml für Toxin A und 0.05 ng/ml für Toxin B. Andere biologische Assays werden nicht durchgeführt.

    Bezüglich der Antikörper, die wir gegen das Toxin A bzw. B verfügbar haben, habe vermutlich weniger gute Nachrichten für Sie:

    Wir haben derzeit vier anti-Toxin A-Antikörper in unserem Katalog:

    ab35021 und ab93318: Kaninchen-polyklonale, deren Immunogen allerdings C-terminal lokalisiert ist.
    https://www.abcam.com/index.html?datasheet=35021 (or use the following: https://www.abcam.com/index.html?datasheet=35021).
    https://www.abcam.com/index.html?datasheet=93318 (or use the following: https://www.abcam.com/index.html?datasheet=93318).

    ab19953 und ab82285: Maus-monoklonale, die gegen das Volllängenprotein generiert wurden. Das Epitop wurde leider nicht kartiert. Es ist also nicht auszuschließen, dass diese Antikörper den C-Terminus binden und blockieren.
    https://www.abcam.com/index.html?datasheet=19953 (or use the following: https://www.abcam.com/index.html?datasheet=19953).
    https://www.abcam.com/index.html?datasheet=82285 (or use the following: https://www.abcam.com/index.html?datasheet=82285).

    Wir haben derzeit drei anti-Toxin B-Antikörper in unserem Katalog:

    ab77582 und ab77583: Maus-monoklonale (wie oben), die gegen das Volllängenprotein generiert wurden. Das Epitop wurde leider nicht kartiert. Es ist also nicht auszuschließen, dass diese Antikörper den C-Terminus binden und blockieren.
    https://www.abcam.com/index.html?datasheet=77582 (or use the following: https://www.abcam.com/index.html?datasheet=77582).
    https://www.abcam.com/index.html?datasheet=77583 (or use the following: https://www.abcam.com/index.html?datasheet=77583).

    ab83066: Kaninchen-polyklonaler, der gegen das N-terminale 60 kDa katalytische Fragment generiert wurde.
    https://www.abcam.com/index.html?datasheet=83066 (or use the following: https://www.abcam.com/index.html?datasheet=83066).

    Ist da vielleicht einer (ab83066?) dabei, der für Ihre Versuchsvorhaben in Frage käme?

    Nähere Informationen sowie die getesteten Anwendungen und Spezies können Sie auf unseren Produktdatenblättern einsehen. Wir garantieren, dass das Produkt so funktioniert wieauf dem Datenblatt steht (https://www.abcam.com/Abpromise).

    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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