Anti-Clostridium difficile Toxin B antibody (ab83066)

Abreviews (2)Q&A (5)

Overview

  • Product name
    Anti-Clostridium difficile Toxin B antibody
  • Description
    Rabbit polyclonal to Clostridium difficile Toxin B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    N-terminal 60 kDa catalytic fragment of Clostridium difficile Toxin B from E. coli.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.

Target

  • Relevance
    Clostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli.
  • Cellular localization
    Secreted
  • Alternative names
    • C. difficile Toxin B antibody
    • Clostridium difficile Toxin B antibody
    • Cytotoxin B antibody
    • tcdB antibody
    • toxB antibody
    • Toxin B antibody
    see all

References

ab83066 has not yet been referenced specifically in any publications.

Publishing research using ab83066? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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7 reviews or Q&A

10
Votes: Highest First

Flow Cytometry abreview for Anti-Clostridium difficile Toxin B antibody

 Excellent
Abreviews
Application
Flow Cytometry
Sample
Bacteria Cell (Mouse fecal bacteria)
Permeabilization
No
Gating Strategy
Bacterias which binding to C.diff toxin B
Specification
Mouse fecal bacteria
Preparation
Cell harvesting/tissue preparation method: Approximately 50mg of formaldehyde-fixed fecal material was homogenized in 10ml of sterile,non-bacteriostatic PBS.The slurry was then passed through 0.25mm stainless steel laboratory sieve for 3 times.The resulting material was centrifuged at 6000*g for 15min.The resulting concentrated fecal bacteria was resuspended to a final concentration of 1*10^8 CFU/ml.
Sample buffer: Phosphate Buffer solution
Fixation
Formaldehyde
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Qianyun Lin

Verified customer

Submitted Sep 20 2018

Western blot abreview for Anti-Clostridium difficile Toxin B antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Clostridium difficile Purified protein (Purified toxin B from Clostridium difficile supern)
Gel Running Conditions
Non-reduced Denaturing (3-8% Tris-Acetate Gel)
Loading amount
10 µg
Specification
Purified toxin B from Clostridium difficile supern
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Qianyun Lin

Verified customer

Submitted Sep 21 2018

1) Proteine (Toxine A+B): Welcher C.difficile-Stamm? Aktivitaet?
2) Antikoerper dagegen, die NICHT am C-Terminus binden?

Vielen Dank für Ihren Anruf.

Zu den Toxinen kann ich Ihnen folgende Informationen aus dem Labor weiterleiten:

Clostridium difficile Toxin A Protein ab123999und Clostridium difficile Toxin B Protein ab124001werden aus demClostri...

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I am considering ordering anti-TcdB antibody from you but note in the western guide you suggest using “ECL+”. Can you tell me who supplies this, presumably enhanced version of ECL?

Thank you for contacting us.

Unfortunately, I cannot tell you from which supplier our lab receives they ECL reagent, but I found a very interesting site from GE healthcare, on which they compare a lot of different substrates as well as provi...

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Bonjour
Je suis désolée pour le retard.
merci pour vos réponses. Concernant la facture, on avait pas encore payé et la société nous a envoyé un courrier donc on va leur renvoyer un courrier ave...

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Merci pour votre réponse.

Je suis désolé que nous n'ayons pas pu vous fournir un bon anti-Clostridium difficile Toxin B et j'espère que vous trouverez un autre fournisseur pour ce type d'anticorps.

J'ai d...

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WB : beaucoup de bruit de fond. pas de bande pour CdTB avec ab83066

Merci de nous avoir contactés. Nous sommes désolés que le produit ab83066 que vous avez reçu ne fonctionne pas comme attendu. Comme convenu j'ai procédé à l'envoi d'une unité gratuite de ab77583. ...

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Dear Abcam,   I have purchsed a number of clostridium Ab from you recently and am writing to request further information on the the protein and antibodies.   For ab83066 do you have the sequence of the protein.  has this antibody been raise...

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Thank you for your inquiry. ab83066: To generate the antisera for this antibody a recombinant GST-fused N-terminal 60 kDa catalytic fragment (which showed glycosylation activity with Rac and Rho) was used. I am sorry we do not have any info...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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