Anti-Clostridium difficile Toxin B antibody (ab83066)

Overview

  • Product name
    Anti-Clostridium difficile Toxin B antibody
  • Description
    Rabbit polyclonal to Clostridium difficile Toxin B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    N-terminal 60 kDa catalytic fragment of Clostridium difficile Toxin B from E. coli.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.

Target

  • Relevance
    Clostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli.
  • Cellular localization
    Secreted
  • Alternative names
    • C. difficile Toxin B antibody
    • Clostridium difficile Toxin B antibody
    • Cytotoxin B antibody
    • tcdB antibody
    • toxB antibody
    • Toxin B antibody
    see all

References

ab83066 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Bacteria Cell (Mouse fecal bacteria)
Permeabilization
No
Gating Strategy
Bacterias which binding to C.diff toxin B
Specification
Mouse fecal bacteria
Preparation
Cell harvesting/tissue preparation method: Approximately 50mg of formaldehyde-fixed fecal material was homogenized in 10ml of sterile,non-bacteriostatic PBS.The slurry was then passed through 0.25mm stainless steel laboratory sieve for 3 times.The resulting material was centrifuged at 6000*g for 15min.The resulting concentrated fecal bacteria was resuspended to a final concentration of 1*10^8 CFU/ml.
Sample buffer: Phosphate Buffer solution
Fixation
Formaldehyde
Username

Qianyun Lin

Verified customer

Submitted Sep 20 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Clostridium difficile Purified protein (Purified toxin B from Clostridium difficile supern)
Gel Running Conditions
Non-reduced Denaturing (3-8% Tris-Acetate Gel)
Loading amount
10 µg
Specification
Purified toxin B from Clostridium difficile supern
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Qianyun Lin

Verified customer

Submitted Sep 21 2018

Vielen Dank für Ihren Anruf.

Zu den Toxinen kann ich Ihnen folgende Informationen aus dem Labor weiterleiten:

Clostridium difficile Toxin A Protein ab123999und Clostridium difficile Toxin B Protein ab124001werden aus demClostri...

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Thank you for contacting us.

Unfortunately, I cannot tell you from which supplier our lab receives they ECL reagent, but I found a very interesting site from GE healthcare, on which they compare a lot of different substrates as well as provi...

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Merci pour votre réponse.

Je suis désolé que nous n'ayons pas pu vous fournir un bon anti-Clostridium difficile Toxin B et j'espère que vous trouverez un autre fournisseur pour ce type d'anticorps.

J'ai d...

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Merci de nous avoir contactés. Nous sommes désolés que le produit ab83066 que vous avez reçu ne fonctionne pas comme attendu. Comme convenu j'ai procédé à l'envoi d'une unité gratuite de ab77583. ...

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Thank you for your inquiry. ab83066: To generate the antisera for this antibody a recombinant GST-fused N-terminal 60 kDa catalytic fragment (which showed glycosylation activity with Rac and Rho) was used. I am sorry we do not have any info...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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