Anti-Clostridium difficile Toxin B antibody (ab83066)

Reviews (2)Q&A (5)References (1)

Key features and details

  • Rabbit polyclonal to Clostridium difficile Toxin B
  • Suitable for: WB
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Clostridium difficile Toxin B antibody [EPR23357-19] - BSA and Azide free (ab270795)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Clostridium difficile Toxin B antibody
    See all Clostridium difficile Toxin B primary antibodies

  • Description

    Rabbit polyclonal to Clostridium difficile Toxin B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Immunogen

    N-terminal 60 kDa catalytic fragment of Clostridium difficile Toxin B from E. coli.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Preservative: None
  • Concentration information loading...

  • Purity

    Whole antiserum

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab83066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000.
Notes
WB
1/1000.

Target

  • Relevance

    Clostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli.

  • Cellular localization

    Secreted

  • Alternative names

    • C. difficile Toxin B antibody
    • Clostridium difficile Toxin B antibody
    • Cytotoxin B antibody
    • tcdB antibody
    • toxB antibody
    • Toxin B antibody
    see all

References (1)

Publishing research using ab83066? Please let us know so that we can cite the reference in this datasheet.

ab83066 has been referenced in 1 publication.

  • Walker DG  et al. Patterns of Expression of Purinergic Receptor P2RY12, a Putative Marker for Non-Activated Microglia, in Aged and Alzheimer's Disease Brains. Int J Mol Sci 21:N/A (2020). PubMed: 31968618

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-Clostridium difficile Toxin B antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Clostridium difficile Purified protein (Purified toxin B from Clostridium difficile supern)
Gel Running Conditions
Non-reduced Denaturing (3-8% Tris-Acetate Gel)
Loading amount
10 µg
Specification
Purified toxin B from Clostridium difficile supern
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Qianyun Lin

Verified customer

Submitted Sep 21 2018

Flow Cytometry abreview for Anti-Clostridium difficile Toxin B antibody

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Bacteria Cell (Mouse fecal bacteria)
Permeabilization
No
Gating Strategy
Bacterias which binding to C.diff toxin B
Specification
Mouse fecal bacteria
Preparation
Cell harvesting/tissue preparation method: Approximately 50mg of formaldehyde-fixed fecal material was homogenized in 10ml of sterile,non-bacteriostatic PBS.The slurry was then passed through 0.25mm stainless steel laboratory sieve for 3 times.The resulting material was centrifuged at 6000*g for 15min.The resulting concentrated fecal bacteria was resuspended to a final concentration of 1*10^8 CFU/ml.
Sample buffer: Phosphate Buffer solution
Fixation
Formaldehyde
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Qianyun Lin

Verified customer

Submitted Sep 20 2018

Question

1) Proteine (Toxine A+B): Welcher C.difficile-Stamm? Aktivitaet?
2) Antikoerper dagegen, die NICHT am C-Terminus binden?

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Answer

Vielen Dank für Ihren Anruf.

Zu den Toxinen kann ich Ihnen folgende Informationen aus dem Labor weiterleiten:

Clostridium difficile Toxin A Protein ab123999und Clostridium difficile Toxin B Protein ab124001werden aus demClostridium difficile Stamm VPI10463 aufgereinigt, der Toxinotyp 0-Toxine produziert.

Beide Toxine sind aktiv; jeder Batch wird im Verocytotoxicity Assay getestet und ist toxisch für Zellen bis zu einer Konzentration von mindestens5 ng/ml für Toxin A und 0.05 ng/ml für Toxin B. Andere biologische Assays werden nicht durchgeführt.

Bezüglich der Antikörper, die wir gegen das Toxin A bzw. B verfügbar haben, habe vermutlich weniger gute Nachrichten für Sie:

Wir haben derzeit vier anti-Toxin A-Antikörper in unserem Katalog:

ab35021 und ab93318: Kaninchen-polyklonale, deren Immunogen allerdings C-terminal lokalisiert ist.
https://www.abcam.com/Clostridium-difficile-Toxin-A-antibody-ab35021.html (or use the following: https://www.abcam.com/Clostridium-difficile-Toxin-A-antibody-ab35021.html).
https://www.abcam.com/Clostridium-difficile-Toxin-A-antibody-ab93318.html (or use the following: https://www.abcam.com/Clostridium-difficile-Toxin-A-antibody-ab93318.html).

ab19953 und ab82285: Maus-monoklonale, die gegen das Volllängenprotein generiert wurden. Das Epitop wurde leider nicht kartiert. Es ist also nicht auszuschließen, dass diese Antikörper den C-Terminus binden und blockieren.
https://www.abcam.com/Clostridium-difficile-Toxin-A-antibody-PCG4-ab19953.html (or use the following: https://www.abcam.com/Clostridium-difficile-Toxin-A-antibody-PCG4-ab19953.html).
https://www.abcam.com/Clostridium-difficile-Toxin-A-antibody-B618M-ab82285.html (or use the following: https://www.abcam.com/Clostridium-difficile-Toxin-A-antibody-B618M-ab82285.html).

Wir haben derzeit drei anti-Toxin B-Antikörper in unserem Katalog:

ab77582 und ab77583: Maus-monoklonale (wie oben), die gegen das Volllängenprotein generiert wurden. Das Epitop wurde leider nicht kartiert. Es ist also nicht auszuschließen, dass diese Antikörper den C-Terminus binden und blockieren.
https://www.abcam.com/Clostridium-difficile-Toxin-B-antibody-B426M-ab77582.html (or use the following: https://www.abcam.com/Clostridium-difficile-Toxin-B-antibody-B426M-ab77582.html).
https://www.abcam.com/Clostridium-difficile-Toxin-B-antibody-B428M-ab77583.html (or use the following: https://www.abcam.com/Clostridium-difficile-Toxin-B-antibody-B428M-ab77583.html).

ab83066: Kaninchen-polyklonaler, der gegen das N-terminale 60 kDa katalytische Fragment generiert wurde.
https://www.abcam.com/Clostridium-difficile-Toxin-B-antibody-ab83066.html (or use the following: https://www.abcam.com/Clostridium-difficile-Toxin-B-antibody-ab83066.html).

Ist da vielleicht einer (ab83066?) dabei, der für Ihre Versuchsvorhaben in Frage käme?

Nähere Informationen sowie die getesteten Anwendungen und Spezies können Sie auf unseren Produktdatenblättern einsehen. Wir garantieren, dass das Produkt so funktioniert wieauf dem Datenblatt steht (https://www.abcam.com/Abpromise).

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question

I am considering ordering anti-TcdB antibody from you but note in the western guide you suggest using “ECL+”. Can you tell me who supplies this, presumably enhanced version of ECL?

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Answer

Thank you for contacting us.

Unfortunately, I cannot tell you from which supplier our lab receives they ECL reagent, but I found a very interesting site from GE healthcare, on which they compare a lot of different substrates as well as providing them. The address is as follow:

http://www.gelifesciences.com/aptrix/upp01077.nsf/content/lsn_online_article_190503_d

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Bonjour
Je suis désolée pour le retard.
merci pour vos réponses. Concernant la facture, on avait pas encore payé et la société nous a envoyé un courrier donc on va leur renvoyer un courrier avec une photo du Western qui n'a pas fonctionné (premier anticorps).
Merci
Cordialement

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Answer

Merci pour votre réponse.

Je suis désolé que nous n'ayons pas pu vous fournir un bon anti-Clostridium difficile Toxin B et j'espère que vous trouverez un autre fournisseur pour ce type d'anticorps.

J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN. Inutile de leur envoyer une image du blot.

Votre référence CN est *******.

Voici les manières dont vous pouvez utiliser cette référence CN:
(1) la faire valoir contre la facture originale si celle-ci n'as pas encore été payée
(2) la faire valoir contre une prochaine facture
(3) obtenir un remboursement de la somme entière si vous n'avez pas de factures actuellement en cours avec Abcam

Si vous souhaitez recevoir un remboursement au lieu d'un avoir, veuillez demander à votre service de gestion de prendre contact avec notre département comptabilité afin que nous puissions recueillir les informations nécessaires pour cette restitution. Notre service comptabilité peut être contacté par courriel à l'adresse creditcontrol@abcam.com ou par téléphone en utilisant le lien « Contactez-nous » dans le coin en haut à droite de notre site internet. Veuillez communiquer votre numéro d'avoir lorsque vous contactez notre service comptabilité.

Vous recevrez également la référence complète de la note de crédit par courriel ou par voie postale; cette référence commençant par les lettres CGB.

J'espère que cette expérience ne vous empêchera pas de commander à nouveau l'un de nos produits.

L'équipe technique reste à votre disposition pour plus de conseils.

Bonne continuation dans votre recherche.

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Question

WB : beaucoup de bruit de fond. pas de bande pour CdTB avec ab83066

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Answer

Merci de nous avoir contactés. Nous sommes désolés que le produit ab83066 que vous avez reçu ne fonctionne pas comme attendu. Comme convenu j'ai procédé à l'envoi d'une unité gratuite de ab77583. Le numéro de commande de remplacement gratuit de ce produit est ********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. N'hésitez pas à nous communiquer vos résultats en Western Blot avec cet anticorpsen utilisant notre système d'Abreview : https://www.abcam.com/index.html?pageconfig=your_reviews&intAbID=77583&strAccessMode=public_create

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Question

Dear Abcam,   I have purchsed a number of clostridium Ab from you recently and am writing to request further information on the the protein and antibodies.   For ab83066 do you have the sequence of the protein.  has this antibody been raised against the entire 60 kDa N terminal region (if so which strain is this based on) or against a fragement/region of the N terminal. do you have any details on the production of the recombinant  fragment and how it was produced in E.coli.   For ab77582-  can you provide any further information on the antibody, what it recognises how it was produced.   Thanking you for your help  

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Answer

Thank you for your inquiry. ab83066: To generate the antisera for this antibody a recombinant GST-fused N-terminal 60 kDa catalytic fragment (which showed glycosylation activity with Rac and Rho) was used. I am sorry we do not have any information about which strain it was based on. ab77582: Unfortunately, we do not have information about the actual antibody binding site. The epitope was not mapped. But I can confirm that this antibody is specific to Clostridium difficile Toxin B and will not cross react with Clostridium difficile Toxin A. I hope this information was helpful. Please do not hesitate to contact me again with any further questions.

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