Recombinant Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)
Overview
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Product name
Anti-Clostridium difficile Toxin B antibody [EPR23357-19]
See all Clostridium difficile Toxin B primary antibodies -
Description
Rabbit monoclonal [EPR23357-19] to Clostridium difficile Toxin B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Clostridium difficile -
Immunogen
Recombinant fragment within Clostridium difficile Toxin B aa 1-550. The exact sequence is proprietary. Clostridioides difficile
Database link: P18177 -
Positive control
- WB: Native Clostridium difficile Toxin B protein (ab124001) 10ng
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23357-19 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
Our Abpromise guarantee covers the use of ab270452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 308 kDa (predicted molecular weight: 270 kDa). |
Target
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Relevance
Clostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli. -
Cellular localization
Secreted -
Database links
- SwissProt: P18177 Clostridium difficile
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Alternative names
- C. difficile Toxin B antibody
- Clostridium difficile Toxin B antibody
- Cytotoxin B antibody
see all
Images
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![Western blot - Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)](https://www.abcam.com/ps/products/270/ab270452/Images/ab270452-384763-anti-clostridium-difficile-toxin-b-antibody-epr2335719-western-blot-native-clostridium-difficile-toxin-a-protein-native-clostridium-difficile-toxin-b-protein-lysates.jpg)
Western blot - Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)All lanes : Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452) at 1/1000 dilution
Lane 1 : Native Clostridium difficile Toxin A protein (ab123999) 10ng
Lane 2 : Native Clostridium difficile Toxin B protein (ab124001) 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 270 kDa
Observed band size: 308 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Both the recombinant proteins were from Abcam.
Exposure time: lane 1: 3 minutes lane 2: 15 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab270452 has not yet been referenced specifically in any publications.
