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RecombinantRabMAb

Recombinant Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)

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Western blot - Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)
  • Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23357-19] to Clostridium difficile Toxin B
  • Suitable for: WB
  • Reacts with: Clostridium difficile

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Clostridium difficile Toxin B antibody [EPR23357-19]
    See all Clostridium difficile Toxin B primary antibodies

  • Description

    Rabbit monoclonal [EPR23357-19] to Clostridium difficile Toxin B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Clostridium difficile

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Native Clostridium difficile Toxin B protein (ab124001) 10ng

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23357-19

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 308 kDa (predicted molecular weight: 270 kDa).
Notes
WB
1/1000. Detects a band of approximately 308 kDa (predicted molecular weight: 270 kDa).

Target

  • Relevance

    Clostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • C. difficile Toxin B antibody
      • Clostridium difficile Toxin B antibody
      • Cytotoxin B antibody
      • tcdB antibody
      • toxB antibody
      • Toxin B antibody
      see all

    Images

    • Western blot - Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)
      Western blot - Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)
      All lanes : Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452) at 1/1000 dilution

      Lane 1 : Native Clostridium difficile Toxin A protein (ab123999) 10ng
      Lane 2 : Native Clostridium difficile Toxin B protein (ab124001) 10ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 270 kDa
      Observed band size: 308 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Both the recombinant proteins were from Abcam.

      Exposure time: lane 1: 3 minutes lane 2: 15 seconds

    • Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)
      Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab270452? Please let us know so that we can cite the reference in this datasheet.

    ab270452 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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