Product nameAnti-Clostridium difficile Toxin B antibody [EPR23357-19]
See all Clostridium difficile Toxin B primary antibodies
DescriptionRabbit monoclonal [EPR23357-19] to Clostridium difficile Toxin B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Clostridium difficile
Recombinant fragment within Clostridium difficile Toxin B aa 1-550. The exact sequence is proprietary. Clostridioides difficile
Database link: P18177
- WB: Native Clostridium difficile Toxin B protein (ab124001) 10ng
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab270452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 308 kDa (predicted molecular weight: 270 kDa).|
RelevanceClostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli.
- SwissProt: P18177 Clostridium difficile
- C. difficile Toxin B antibody
- Clostridium difficile Toxin B antibody
- Cytotoxin B antibody
All lanes : Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452) at 1/1000 dilution
Lane 1 : Native Clostridium difficile Toxin A protein (ab123999) 10ng
Lane 2 : Native Clostridium difficile Toxin B protein (ab124001) 10ng
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 270 kDa
Observed band size: 308 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Both the recombinant proteins were from Abcam.
Exposure time: lane 1: 3 minutes lane 2: 15 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270452 has not yet been referenced specifically in any publications.