Key features and details
- Non-selective P450 cytochrome inhibitor
- CAS Number: 23593-75-1
- Purity: > 99%
- Soluble in DMSO to 75 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Product nameClotrimazole, Non-selective P450 cytochrome inhibitor
DescriptionNon-selective P450 cytochrome inhibitor
Non-selective P450 cytochrome inhibitor (Ki = 0.02-67 μM for P450 cyctochrome subtypes). Potent inhibitor of 34A (Ki = 20 nM). Antifungal agent.
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 75 mM and in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
2D chemical structure image of ab120744, Clotrimazole, Non-selective P450 cytochrome inhibitor
HepG2 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of clotrimazole (ab120744)in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120744 has not yet been referenced specifically in any publications.