Overview

  • Product name

    Clotrimazole, Non-selective P450 cytochrome inhibitor
  • Description

    Non-selective P450 cytochrome inhibitor
  • Alternative names

    • CLT
  • Biological description

    Non-selective P450 cytochrome inhibitor (Ki = 0.02-67 μM for P450 cyctochrome subtypes). Potent inhibitor of 34A (Ki = 20 nM). Antifungal agent.
  • Purity

    > 99%
  • CAS Number

    23593-75-1
  • Chemical structure

    Chemical Structure

Properties

Images

  • HepG2 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of clotrimazole (ab120744)in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.

References

This product has been referenced in:

  • Zhang W  et al. Inhibition of cytochromes P450 by antifungal imidazole derivatives. Drug Metab Dispos 30:314-8 (2002). Read more (PubMed: 11854151) »
  • Thomas GP  et al. The antifungal antibiotic clotrimazole potently inhibits L-type calcium current in guinea-pig ventricular myocytes. Br J Pharmacol 126:1531-3 (1999). Read more (PubMed: 10323582) »
  • Vanden Bossche H  et al. Biochemical basis for the activity and selectivity of oral antifungal drugs. Br J Clin Pract Suppl 71:41-6 (1990). Read more (PubMed: 2091733) »

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