Recombinant Anti-CLP1 antibody [EPR7180(2)] (ab133669)
![Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669)](https://www.abcam.com/ps/products/133/ab133669/Images/ab133669-147639-anti-heab-antibody-epr71802-western-blot.jpg "Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7180(2)] to CLP1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CLP1 antibody [EPR7180(2)]
See all CLP1 primary antibodies -
Description
Rabbit monoclonal [EPR7180(2)] to CLP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
corresponding to Human CLP1 (N terminal).
Database link: Q92989 -
Positive control
- HeLa, HepG2 and 293T cell lysates; Human colon and tonsil tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as HEAB
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7180(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab133669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 48 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. -
Sequence similarities
Belongs to the Clp1 family. Clp1 subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10978 Human
- Omim: 608757 Human
- SwissProt: Q92989 Human
- Unigene: 523687 Human
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Alternative names
- ATP/GTP binding protein antibody
- Cleavage and polyadenylation factor I subunit 1 antibody
- CLP 1 antibody
see all
Images
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Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669)All lanes : Anti-CLP1 antibody [EPR7180(2)] (ab133669) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 48 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)](https://www.abcam.com/ps/products/133/ab133669/Images/ab133669-147638-anti-heab-antibody-epr71802-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling CLP1 with ab133669 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)](https://www.abcam.com/ps/products/133/ab133669/Images/ab133669-147637-anti-heab-antibody-epr71802-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CLP1 with ab133669 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (ab133669)](https://www.abcam.com/ps/products/133/ab133669/Images/ab133669-147636-anti-heab-antibody-epr71802.jpg)
OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (ab133669) -
![Anti-CLP1 antibody [EPR7180(2)] (ab133669)](https://www.abcam.com/ps/products/133/ab133669/Images/ab133669-5-benefits-of-recombinant-antibodies.png)
Anti-CLP1 antibody [EPR7180(2)] (ab133669)
Protocols
References (1)
ab133669 has been referenced in 1 publication.
- Schaffer AE et al. CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration. Cell 157:651-63 (2014). WB, IF . PubMed: 24766810
