For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    clp1-antibody-epr71802-ab133669.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
Share by email
RecombinantRabMAb

Recombinant Anti-CLP1 antibody [EPR7180(2)] (ab133669)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
  • OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
  • Anti-CLP1 antibody [EPR7180(2)] (ab133669)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7180(2)] to CLP1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-CLP1 antibody [EPR7180(2)]
    See all CLP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7180(2)] to CLP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human CLP1 (N terminal).
    Database link: Q92989

  • Positive control

    • HeLa, HepG2 and 293T cell lysates; Human colon and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as HEAB

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 8.50 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7180(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • RNAi
    • RISC

Associated products

  • Alternative Versions

    • Anti-CLP1 antibody [EPR7180(2)] - BSA and Azide free (ab248605)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HepG2 nuclear extract lysate (ab14660)

Applications

Our Abpromise guarantee covers the use of ab133669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
    • Sequence similarities

      Belongs to the Clp1 family. Clp1 subfamily.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q92989 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10978 Human
      • Omim: 608757 Human
      • SwissProt: Q92989 Human
      • Unigene: 523687 Human
      • Alternative names

        • ATP/GTP binding protein antibody
        • Cleavage and polyadenylation factor I subunit 1 antibody
        • CLP 1 antibody
        • clp1 antibody
        • CLP1 cleavage and polyadenylation factor I subunit homolog antibody
        • CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) antibody
        • CLP1, yeast, homolog of antibody
        • CLP1_HUMAN antibody
        • hClp1 antibody
        • Homolog of yeast CFIA subunit Clp1p antibody
        • Polyadenylation factor Clp1 antibody
        • Polynucleotide kinase Clp1 antibody
        • Polyribonucleotide 5' hydroxyl kinase Clp1 antibody
        • Polyribonucleotide 5''-hydroxyl-kinase Clp1 antibody
        • Pre mRNA cleavage complex II protein Clp1 antibody
        • Pre-mRNA cleavage complex II protein Clp1 antibody
        see all

      Images

      • Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
        Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
        All lanes : Anti-CLP1 antibody [EPR7180(2)] (ab133669) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

        Predicted band size: 48 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)

        Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling CLP1 with ab133669 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)

        Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CLP1 with ab133669 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
        OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-CLP1 antibody [EPR7180(2)] (ab133669)
        Anti-CLP1 antibody [EPR7180(2)] (ab133669)

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab133669? Please let us know so that we can cite the reference in this datasheet.

      ab133669 has been referenced in 1 publication.

      • Schaffer AE  et al. CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration. Cell 157:651-63 (2014). WB, IF . PubMed: 24766810

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab133669.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.