Overview

  • Product name

    Anti-CLP1 antibody [EPR7181]
    See all CLP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7181] to CLP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CLP1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • HeLa, HepG2, 293T and Jurkat whole cell lysate (ab7899); Jurkat cells
  • General notes

     

     

     This product was previously labelled as HEAB

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
    • Sequence similarities

      Belongs to the Clp1 family. Clp1 subfamily.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP/GTP binding protein antibody
      • Cleavage and polyadenylation factor I subunit 1 antibody
      • CLP 1 antibody
      • clp1 antibody
      • CLP1 cleavage and polyadenylation factor I subunit homolog antibody
      • CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) antibody
      • CLP1, yeast, homolog of antibody
      • CLP1_HUMAN antibody
      • hClp1 antibody
      • Homolog of yeast CFIA subunit Clp1p antibody
      • Polyadenylation factor Clp1 antibody
      • Polynucleotide kinase Clp1 antibody
      • Polyribonucleotide 5' hydroxyl kinase Clp1 antibody
      • Polyribonucleotide 5''-hydroxyl-kinase Clp1 antibody
      • Pre mRNA cleavage complex II protein Clp1 antibody
      • Pre-mRNA cleavage complex II protein Clp1 antibody
      see all

    Images

    • All lanes : Anti-CLP1 antibody [EPR7181] (ab128939) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

    • ab128939 at 1/100 staining CLP1 in permeabilized Jurkat cells by flow cytometry (red), control Rabbit IgG (green).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Khleborodova A  et al. An investigation into the role of ATP in the mammalian pre-mRNA 3' cleavage reaction. Biochimie 125:213-22 (2016). Read more (PubMed: 27060432) »
    See 1 Publication for this product

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