Key features and details
- Rabbit polyclonal to CLPB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CLPB antibody
See all CLPB primary antibodies
DescriptionRabbit polyclonal to CLPB
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human CLPB aa 1-350.
Database link: Q9H078
- WB: HEK-293T, COLO205 and MCF7 whole cell lysate; Mouse brain tissue lysate. IHC-P: Human adrenal gland tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionMay function as a regulatory ATPase and be related to secretion/protein trafficking process.
Sequence similaritiesBelongs to the clpA/clpB family.
Contains 4 ANK repeats.
- Information by UniProt
- Caseinolytic peptidase B antibody
- Caseinolytic peptidase B protein homolog antibody
- clpB antibody
Paraffin-embedded human adrenal gland tissue stained for CLPB using ab235349 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-CLPB antibody (ab235349) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : COLO205 (Human colon adenocarcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235349 has been referenced in 1 publication.
- Cupo RR & Shorter J Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations. Elife 9:N/A (2020). PubMed: 32573439