Anti-CLPB antibody (ab235349)
Key features and details
- Rabbit polyclonal to CLPB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CLPB antibody
See all CLPB primary antibodies -
Description
Rabbit polyclonal to CLPB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human CLPB aa 1-350.
Database link: Q9H078 -
Positive control
- WB: HEK-293T, COLO205 and MCF7 whole cell lysate; Mouse brain tissue lysate. IHC-P: Human adrenal gland tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/2000. |
IHC-P
1/20 - 1/200. |
Target
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Function
May function as a regulatory ATPase and be related to secretion/protein trafficking process. -
Sequence similarities
Belongs to the clpA/clpB family.
Contains 4 ANK repeats. - Information by UniProt
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Database links
- Entrez Gene: 81570 Human
- Entrez Gene: 20480 Mouse
- SwissProt: Q9H078 Human
- SwissProt: Q60649 Mouse
- Unigene: 523877 Human
- Unigene: 3990 Mouse
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Alternative names
- Caseinolytic peptidase B antibody
- Caseinolytic peptidase B protein homolog antibody
- clpB antibody
see all
Images
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Paraffin-embedded human adrenal gland tissue stained for CLPB using ab235349 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-CLPB antibody (ab235349) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : COLO205 (Human colon adenocarcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab235349 has been referenced in 1 publication.
- Cupo RR & Shorter J Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations. Elife 9:N/A (2020). PubMed: 32573439