Recombinant
RabMAb

Recombinant Anti-CLPP antibody [EPR7133] (ab124822)

Overview

  • Product name

    Anti-CLPP antibody [EPR7133]
    See all CLPP primary antibodies
  • Description

    Rabbit monoclonal [EPR7133] to CLPP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CLPP.

  • Positive control

    • WB: Recombinant Human CLPP protein (ab124872), Fetal muscle, Saos-2, A431, 293T, K562, A549, and Mouse brain lysates; IHC-P: Human liver, Mouse kidney, and Rat stomach tissues IP: K562
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
    • Tissue specificity

      Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney.
    • Sequence similarities

      Belongs to the peptidase S14 family.
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP dependent protease ClpAP (E coli) proteolytic subunit antibody
      • ATP dependent protease ClpAP proteolytic subunit antibody
      • ATP dependent protease ClpAP proteolytic subunit human antibody
      • ATP dependent proteolytic subunit homolog (E coli) antibody
      • ATP dependent proteolytic subunit homolog antibody
      • Caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
      • Caseinolytic protease ATP dependent proteolytic subunit E coli antibody
      • clpP antibody
      • ClpP caseinolytic peptidase ATP dependent proteolytic subunit antibody
      • ClpP caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
      • CLPP_HUMAN antibody
      • Endopeptidase Clp antibody
      • mitochondrial antibody
      • Putative ATP dependent Clp protease proteolytic subunit mitochondrial antibody
      • Putative ATP dependent Clp protease proteolytic subunit antibody
      • Putative ATP-dependent Clp protease proteolytic subunit antibody
      see all

    Images

    • All lanes : Anti-CLPP antibody [EPR7133] (ab124822) at 0.05 µg/ml (purified)

      Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
      Lane 3 : Mouse brain lysates
      Lane 4 : Rat brain lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 30 kDa



      Blocking and diluting buffer: 5% NFDM/TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
    • ab124822 (purified) at 1:20 dilution (2µg) immunoprecipitating CLPP in K562 whole cell lysate.
      Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
      Lane 2 (+): ab124822 & K562 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124822 in K562 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-CLPP antibody [EPR7133] (ab124822) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal muscle lysate
      Lane 2 : Saos-2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : K562 cell lysate
      Lane 6 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 30 kDa
      Observed band size: 26 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Nakazawa H  et al. Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle. FEBS Open Bio 9:348-363 (2019). Read more (PubMed: 30761259) »
    • Fu Z  et al. Mutant huntingtin inhibits the mitochondrial unfolded protein response by impairing ABCB10 mRNA stability. Biochim Biophys Acta Mol Basis Dis 1865:1428-1435 (2019). Read more (PubMed: 30802639) »
    See all 16 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Rat Cell lysate - other (ST14a Whole Cell Lysate)
    Gel Running Conditions
    Reduced Denaturing (4-12% SDS-PAGE, Laemmli Buffer with 10% β-Mercaptoethanol,10 min at 55C denaturation.)
    Loading amount
    15 µg
    Specification
    ST14a Whole Cell Lysate
    Blocking step
    SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted May 23 2018

    Answer

    Thank you for contacting Abcam regarding ab124822.


    Regarding the use of this antibody in ICC or IHC-P, this antibody failed to react in these applications during routine QC testing. It is possible that with further troubleshooting the antibody may work, but we do not guarantee that and the product is not covered under our Abpromise for these applications. Similarly, we do not expect the antibody to work for IFC either. If you are working with human samples, I can recommend ab15708 as a suitable alternative.


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up