Recombinant
RabMAb

Recombinant Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)

Overview

  • Product name

    Anti-CLPP antibody [EPR7133] - BSA and Azide free
    See all CLPP primary antibodies
  • Description

    Rabbit monoclonal [EPR7133] to CLPP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CLPP. The exact sequence is proprietary.

  • Positive control

    • WB: Fetal muscle lysate; Saos-2, A431, HEK-293T, K562 and A549 cell lysates.
  • General notes

    Ab236064 is the carrier-free version of ab124822. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).

Target

  • Function

    Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
  • Tissue specificity

    Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney.
  • Sequence similarities

    Belongs to the peptidase S14 family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent protease ClpAP (E coli) proteolytic subunit antibody
    • ATP dependent protease ClpAP proteolytic subunit antibody
    • ATP dependent protease ClpAP proteolytic subunit human antibody
    • ATP dependent proteolytic subunit homolog (E coli) antibody
    • ATP dependent proteolytic subunit homolog antibody
    • Caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
    • Caseinolytic protease ATP dependent proteolytic subunit E coli antibody
    • clpP antibody
    • ClpP caseinolytic peptidase ATP dependent proteolytic subunit antibody
    • ClpP caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
    • CLPP_HUMAN antibody
    • Endopeptidase Clp antibody
    • mitochondrial antibody
    • Putative ATP dependent Clp protease proteolytic subunit mitochondrial antibody
    • Putative ATP dependent Clp protease proteolytic subunit antibody
    • Putative ATP-dependent Clp protease proteolytic subunit antibody
    see all

Images

  • All lanes : Anti-CLPP antibody [EPR7133] (ab124822) at 1/1000 dilution

    Lane 1 : Fetal muscle lysate
    Lane 2 : Saos-2 (Human osteosarcoma cell line) cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 6 : A549 (Human lung carcinoma cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 30 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

  • ab124822 (purified) at 1:20 dilution (2µg) immunoprecipitating CLPP in K562 whole cell lysate.
    Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab124822 & K562 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124822 in K562 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

References

This product has been referenced in:

  • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). Read more (PubMed: 30765471) »
See 1 Publication for this product

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