Overview

  • Product name

    Anti-CLPTM1 antibody [EPR8800]
    See all CLPTM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8800] to CLPTM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CLPTM1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O96005

  • Positive control

    • HeLa cells and cell lysate, 293T cell lysate, U87 MG cell lysate, HepG2 cell lysate, Jurkat cell lysate, Papillary adenocarcinoma of Human thyroid gland tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.45 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8800
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May play a role in T-cell development.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the CLPTM1 family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cleft lip and palate associated transmembrane protein 1 antibody
      • Cleft lip and palate transmembrane protein 1 antibody
      • CLPT1_HUMAN antibody
      • CLPTM 1 antibody
      • Clptm1 antibody
      • CLPTM1 transmembrane protein antibody
      • HS9 antibody
      • N14 antibody
      see all

    Images

    • All lanes : Anti-CLPTM1 antibody [EPR8800] (ab133756) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : U87 MG cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 76 kDa

    • Immunohistochemical analysis of paraffin embedded papillary adenocarcinoma of Human thyroid gland tissue labelling CLPTM1 with ab133756 at 1/50.
    • Immunofluorescent staining of HeLa cells labelling CLPTM1 with ab133756 at 1/250.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133756 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133756.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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