Product nameAnti-CLPTM1 antibody [EPR8800]
See all CLPTM1 primary antibodies
DescriptionRabbit monoclonal [EPR8800] to CLPTM1
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human CLPTM1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O96005
- HeLa cells and cell lysate, 293T cell lysate, U87 MG cell lysate, HepG2 cell lysate, Jurkat cell lysate, Papillary adenocarcinoma of Human thyroid gland tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.45 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
FunctionMay play a role in T-cell development.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the CLPTM1 family.
- Information by UniProt
- Cleft lip and palate associated transmembrane protein 1 antibody
- Cleft lip and palate transmembrane protein 1 antibody
- CLPT1_HUMAN antibody
All lanes : Anti-CLPTM1 antibody [EPR8800] (ab133756) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U87 MG cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 76 kDa
Immunohistochemical analysis of paraffin embedded papillary adenocarcinoma of Human thyroid gland tissue labelling CLPTM1 with ab133756 at 1/50.
Immunofluorescent staining of HeLa cells labelling CLPTM1 with ab133756 at 1/250.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133756 has not yet been referenced specifically in any publications.