Product nameAnti-CLPTM1 antibody [EPR8800] - BSA and Azide free
See all CLPTM1 primary antibodies
DescriptionRabbit monoclonal [EPR8800] to CLPTM1 - BSA and Azide free
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human CLPTM1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O96005
- WB: HAP1, A549 and HeLa cell lysates.
ab248641 is the carrier-free version of ab133756 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248641 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 3.45 x 10 -11 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab248641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionMay play a role in T-cell development.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the CLPTM1 family.
- Information by UniProt
- Cleft lip and palate associated transmembrane protein 1 antibody
- Cleft lip and palate transmembrane protein 1 antibody
- CLPT1_HUMAN antibody
All lanes : Anti-CLPTM1 antibody [EPR8800] (ab133756) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CLPTM1 knockout HAP1 whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 76 kDa
Observed band size: 76 kDa
ab133756 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in CLPTM1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CLPTM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. Ab133756 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133756).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab248641 has not yet been referenced specifically in any publications.