Overview

  • Product name
    Anti-CLPTM1 antibody [EPR8801]
    See all CLPTM1 primary antibodies
  • Description
    Rabbit monoclonal [EPR8801] to CLPTM1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CLPTM1 aa 650 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: O96005

  • Positive control
    • HeLa, 293T, U87-MG and Jurkat cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8801
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 76 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC.
  • Target

    • Relevance
      CLPTM1 may play a role in T-cell development. A chromosomal translocation involving CLPTM1 is found in a family with cleft lip and palate.
    • Cellular localization
      Membrane; Multi-pass membrane protein.
    • Database links
    • Alternative names
      • Cleft lip and palate associated transmembrane protein 1 antibody
      • CLPTM 1 antibody
      • CLPTM1 transmembrane protein antibody
      • HS9 antibody
      • N14 antibody
      see all

    Images

    • All lanes : Anti-CLPTM1 antibody [EPR8801] (ab174839) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 76 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling CLPTM1 with ab174839 at a 1/100 dilution (red), or a rabbit IgG (negative) (green).

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate, labeling CLPTM1 immunoprecipitated using ab174839 at 1/10 dilution.

    References

    ab174839 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174839.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up