Recombinant
Anti-CLPX antibody [EP8772] (ab168338)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-CLPX antibody [EP8772]
See all CLPX primary antibodies -
DescriptionRabbit monoclonal [EP8772] to CLPX
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to residues in Human CLPX (O76031).
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Positive control
- A673, HT-1080, Saos-2, Human fetal liver & heart lysates, A673 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP8772
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
- Anti-CLPX antibody [EP8772] (HRP) (ab203694)
- Anti-CLPX antibody [EP8772] (Alexa Fluor® 647) (ab203695)
- Anti-CLPX antibody [EP8772] (Alexa Fluor® 488) (ab203976)
- Anti-CLPX antibody [EP8772] (Alexa Fluor® 594) (ab206624)
- Anti-CLPX antibody [EP8772] (Alexa Fluor® 555) (ab206626)
- Anti-CLPX antibody [EP8772] (Alexa Fluor® 568) (ab207274)
- Anti-CLPX antibody [EP8772] - BSA and Azide free (ab240151)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab168338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 69 kDa. | |
| ICC/IF | 1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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FunctionATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.
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Tissue specificityHigher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.
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Sequence similaritiesBelongs to the ClpX chaperone family.
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Cellular localizationMitochondrion.
- Information by UniProt
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Database links
- Entrez Gene: 10845 Human
- Entrez Gene: 270166 Mouse
- Entrez Gene: 300786 Rat
- SwissProt: O76031 Human
- SwissProt: Q9JHS4 Mouse
- SwissProt: Q5U2U0 Rat
- Unigene: 113823 Human
- Unigene: 30088 Mouse
see all -
Alternative names
- ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
- ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
- AU014732 antibody
see all
Images
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![Western blot - Anti-CLPX antibody [EP8772] (ab168338)](https://www.abcam.com/ps/datasheet/images/168/ab168338/CLPX-Primary-antibodies-ab168338-1.jpg)
Western blot - Anti-CLPX antibody [EP8772] (ab168338)All lanes : Anti-CLPX antibody [EP8772] (ab168338) at 1/1000 dilution
Lane 1 : A673 lysates
Lane 2 : HT-1080 lysates
Lane 3 : Saos-2 lysates
Lane 4 : Human fetal liver lysates
Lane 5 : Human fetal heart lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 69 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CLPX antibody [EP8772] (ab168338)](https://www.abcam.com/ps/datasheet/images/168/ab168338/CLPX-Primary-antibodies-ab168338-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CLPX antibody [EP8772] (ab168338)Immunofluorescence analysis of A673 cells (red) labeling CLPX with ab168338 at 1/100 dilution. DAPI nuclear staining (blue).
Datasheets and documents
References
This product has been referenced in:
- Bao Y et al. Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integr Cancer Ther 17:80-91 (2018). Read more (PubMed: 28008780) »
- Franco-Iborra S et al. Defective mitochondrial protein import contributes to complex I-induced mitochondrial dysfunction and neurodegeneration in Parkinson's disease. Cell Death Dis 9:1122 (2018). Read more (PubMed: 30405116) »
