Recombinant Anti-CLPX antibody [EP8772] (ab168338)
![Western blot - Anti-CLPX antibody [EP8772] (ab168338)](https://www.abcam.com/ps/products/168/ab168338/Images/ab168338-166601-anti-clpx-antibody-ep8772-western-blot.jpg "Western blot - Anti-CLPX antibody [EP8772] (ab168338)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP8772] to CLPX
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CLPX antibody [EP8772]
See all CLPX primary antibodies -
Description
Rabbit monoclonal [EP8772] to CLPX -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human CLPX aa 600-700.
Database link: O76031 -
Positive control
- A673, HT-1080, Saos-2, Human fetal liver & heart lysates, A673 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP8772 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
|
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa. |
|
ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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Function
ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP. -
Tissue specificity
Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas. -
Sequence similarities
Belongs to the ClpX chaperone family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 10845 Human
- Entrez Gene: 270166 Mouse
- Entrez Gene: 300786 Rat
- SwissProt: O76031 Human
- SwissProt: Q9JHS4 Mouse
- SwissProt: Q5U2U0 Rat
- Unigene: 113823 Human
- Unigene: 30088 Mouse
see all -
Alternative names
- ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
- ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
- AU014732 antibody
see all
Images
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Western blot - Anti-CLPX antibody [EP8772] (ab168338)All lanes : Anti-CLPX antibody [EP8772] (ab168338) at 1/1000 dilution
Lane 1 : A673 lysates
Lane 2 : HT-1080 lysates
Lane 3 : Saos-2 lysates
Lane 4 : Human fetal liver lysates
Lane 5 : Human fetal heart lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 69 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CLPX antibody [EP8772] (ab168338)](https://www.abcam.com/ps/products/168/ab168338/Images/ab168338-166600-anti-clpx-antibody-ep8772-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CLPX antibody [EP8772] (ab168338)Immunofluorescence analysis of A673 cells (red) labeling CLPX with ab168338 at 1/100 dilution. DAPI nuclear staining (blue). -
![Anti-CLPX antibody [EP8772] (ab168338)](https://www.abcam.com/ps/products/168/ab168338/Images/ab168338-3-benefits-of-recombinant-antibodies.png)
Anti-CLPX antibody [EP8772] (ab168338)
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab168338 has been referenced in 6 publications.
- Maio N et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). PubMed: 30765471
- Hu D et al. Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity. Acta Neuropathol 137:939-960 (2019). PubMed: 30877431
- Huff LP et al. Polymerase-d-interacting protein 2 activates the RhoGEF epithelial cell transforming sequence 2 in vascular smooth muscle cells. Am J Physiol Cell Physiol 316:C621-C631 (2019). PubMed: 30726115
- Bao Y et al. Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integr Cancer Ther 17:80-91 (2018). PubMed: 28008780
- Franco-Iborra S et al. Defective mitochondrial protein import contributes to complex I-induced mitochondrial dysfunction and neurodegeneration in Parkinson's disease. Cell Death Dis 9:1122 (2018). PubMed: 30405116
- Kubota Y et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). PubMed: 27496948
