Overview

  • Product name
    Anti-CLPX antibody [EP8772]
    See all CLPX primary antibodies
  • Description
    Rabbit monoclonal [EP8772] to CLPX
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human CLPX (O76031).

  • Positive control
    • A673, HT-1080, Saos-2, Human fetal liver & heart lysates, A673 cells
  • General notes

     

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.
    • Tissue specificity
      Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.
    • Sequence similarities
      Belongs to the ClpX chaperone family.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
      • ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
      • AU014732 antibody
      • Caseinolytic mitochondrial matrix peptidase chaperone subunit antibody
      • Caseinolytic peptidase X (E.coli) antibody
      • Caseinolytic protease X antibody
      • clpX antibody
      • ClpX caseinolytic peptidase X homolog antibody
      • ClpX caseinolytic protease X homolog antibody
      • CLPX_HUMAN antibody
      • E330029I21 antibody
      • Energy dependent regulator of proteolysis antibody
      see all

    Images

    • All lanes : Anti-CLPX antibody [EP8772] (ab168338) at 1/1000 dilution

      Lane 1 : A673 lysates
      Lane 2 : HT-1080 lysates
      Lane 3 : Saos-2 lysates
      Lane 4 : Human fetal liver lysates
      Lane 5 : Human fetal heart lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 69 kDa

    • Immunofluorescence analysis of A673 cells (red) labeling CLPX with ab168338 at 1/100 dilution. DAPI nuclear staining (blue).

    References

    This product has been referenced in:
    • Bao Y  et al. Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integr Cancer Ther 17:80-91 (2018). Read more (PubMed: 28008780) »
    • Kubota Y  et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). Read more (PubMed: 27496948) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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