Anti-CLPX antibody [EP8772] Recombinant (ab168338)

Product name

Anti-CLPX antibody [EP8772]
See all CLPX primary antibodies
Description

Rabbit monoclonal [EP8772] to CLPX
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide corresponding to residues in Human CLPX (O76031).
Positive control
- A673, HT-1080, Saos-2, Human fetal liver & heart lysates, A673 cells
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP8772
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab168338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
ICC/IF		1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt,IHC-P or IP.

Function

ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.
Tissue specificity

Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.
Sequence similarities

Belongs to the ClpX chaperone family.
Cellular localization

Mitochondrion.
Target information above from: UniProt accession O76031 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10845 Human
- Entrez Gene: 270166 Mouse
- Entrez Gene: 300786 Rat
- SwissProt: O76031 Human
- SwissProt: Q9JHS4 Mouse
- SwissProt: Q5U2U0 Rat
- Unigene: 113823 Human
- Unigene: 30088 Mouse
- Unigene: 203913 Rat
see all
Alternative names
- ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
- ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
- AU014732 antibody
- Caseinolytic mitochondrial matrix peptidase chaperone subunit antibody
- Caseinolytic peptidase X (E.coli) antibody
- Caseinolytic protease X antibody
- clpX antibody
- ClpX caseinolytic peptidase X homolog antibody
- ClpX caseinolytic protease X homolog antibody
- CLPX_HUMAN antibody
- E330029I21 antibody
- Energy dependent regulator of proteolysis antibody
see all

Western blot - Anti-CLPX antibody [EP8772] (ab168338)

All lanes : Anti-CLPX antibody [EP8772] (ab168338) at 1/1000 dilution

Lane 1 : A673 lysates
Lane 2 : HT-1080 lysates
Lane 3 : Saos-2 lysates
Lane 4 : Human fetal liver lysates
Lane 5 : Human fetal heart lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 69 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CLPX antibody [EP8772] (ab168338)

Immunofluorescence analysis of A673 cells (red) labeling CLPX with ab168338 at 1/100 dilution. DAPI nuclear staining (blue).

Bao Y et al. Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integr Cancer Ther 17:80-91 (2018). Read more (PubMed: 28008780) »
Kubota Y et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). Read more (PubMed: 27496948) »

Publishing research using ab168338? Please let us know so that we can cite the reference in this datasheet.

Anti-CLPX antibody [EP8772] (ab168338)