Overview

  • Product name

    Anti-CLPX antibody [EP8772] (HRP)
    See all CLPX primary antibodies
  • Description

    Rabbit monoclonal [EP8772] to CLPX (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CLPX. The exact sequence is proprietary.
    Database link: O76031

  • Positive control

    • WB: HT-1080 and Saos-2 whole cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab203694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

Target

  • Function

    ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.
  • Tissue specificity

    Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.
  • Sequence similarities

    Belongs to the ClpX chaperone family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
    • ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
    • AU014732 antibody
    • Caseinolytic mitochondrial matrix peptidase chaperone subunit antibody
    • Caseinolytic peptidase X (E.coli) antibody
    • Caseinolytic protease X antibody
    • clpX antibody
    • ClpX caseinolytic peptidase X homolog antibody
    • ClpX caseinolytic protease X homolog antibody
    • CLPX_HUMAN antibody
    • E330029I21 antibody
    • Energy dependent regulator of proteolysis antibody
    see all

Images

  • All lanes : Anti-CLPX antibody [EP8772] (HRP) (ab203694) at 1/5000 dilution

    Lane 1 : HT1080 (Human fibrosarcoma cell line) Whole Cell Lysate
    Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 69 kDa


    Exposure time: 12 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203694 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab203694 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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