Product nameAnti-CLPX antibody [EP8772] (HRP)
See all CLPX primary antibodies
DescriptionRabbit monoclonal [EP8772] to CLPX (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human CLPX. The exact sequence is proprietary.
Database link: O76031
- WB: HT-1080 and Saos-2 whole cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab203694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).|
FunctionATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.
Tissue specificityHigher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.
Sequence similaritiesBelongs to the ClpX chaperone family.
- Information by UniProt
- ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
- ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
- AU014732 antibody
All lanes : Anti-CLPX antibody [EP8772] (HRP) (ab203694) at 1/5000 dilution
Lane 1 : HT1080 (Human fibrosarcoma cell line) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 12 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203694 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab203694 has not yet been referenced specifically in any publications.