Western blot abreview for Anti-CLSTN1 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal)
Loading amount
25 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: pbst+1%bsa

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit igG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 90 kDa

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Submitted Aug 27 2012

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