Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2963] to CLSTN1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Product nameAnti-CLSTN1 antibody [EPR2963]
See all CLSTN1 primary antibodies
DescriptionRabbit monoclonal [EPR2963] to CLSTN1
The immunogen used for this product shares 91% homology with CLSTN2. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human CLSTN1 (C terminal). The exact sequence is proprietary.
- Human fetal brain lysate; 293T cell lysate; U87 MG lysate; SH SY5Y lysate and Neuro 2a lysate
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 110 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionInduces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals.
Tissue specificityExpressed in the brain and, a lower level, in the heart, skeletal muscle, kidney and placenta. Accumulates in dystrophic neurites around the amyloid core of Alzheimer disease senile plaques (at protein level).
Sequence similaritiesContains 2 cadherin domains.
DomainThe cytoplasmic domain is involved in interaction with APBA2, as well as the binding of synaptic Ca(2+).
modificationsProteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by presenilin gamma-secretase within the transmembrane domain releases the beta-Alc-alpha chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection. Cell junction > synapse > postsynaptic cell membrane. Nucleus. Neurite tips. Localized in the postsynaptic membrane of both excitatory and inhibitory synapses (By similarity). The AlcICD fragment is translocated to the nucleus upon interaction with APBB1.
- Information by UniProt
- Alc alpha antibody
- Alc-alpha antibody
- Alcadein alpha 1 antibody
Immfluorescent staining of paraffin embedded Human SH SY 5Y cells labelling CLSTN1 with ab134130 at 1/100 dilution
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CLSTN1 with ab134130 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : 293T cell lysate
Lane 3 : U87 MG lysate
Lane 4 : SH SY5Y lysate
Lane 5 : Neuro 2a lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti mouse at 1/2000 dilution
Predicted band size: 110 kDa
Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/200000 dilution + Human fetal brain tissue lysate at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking and diluting buffer: 5% NFDM/TBST
Extra band at: 90kDa We are unsure as to the identity of this extra band.
ab134130 has been referenced in 2 publications.
- Lleó A et al. Changes in Synaptic Proteins Precede Neurodegeneration Markers in Preclinical Alzheimer's Disease Cerebrospinal Fluid. Mol Cell Proteomics 18:546-560 (2019). PubMed: 30606734
- Yu L et al. The Circular RNA Cdr1as Act as an Oncogene in Hepatocellular Carcinoma through Targeting miR-7 Expression. PLoS One 11:e0158347 (2016). PubMed: 27391479