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  1. Link

    clstn1-antibody-epr2963-ab134130.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Amyloid
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RecombinantRabMAb

Recombinant Anti-CLSTN1 antibody [EPR2963] (ab134130)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (ab134130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (ab134130)
  • Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
  • Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
  • Anti-CLSTN1 antibody [EPR2963] (ab134130)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2963] to CLSTN1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-CLSTN1 antibody [EPR2963]
    See all CLSTN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2963] to CLSTN1
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 91%  homology with CLSTN2. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CLSTN1 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal brain lysate; 293T cell lysate; U87 MG lysate; SH SY5Y lysate and Neuro 2a lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2963
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free (ab248693)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CLSTN1 protein (ab182805)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals.
  • Tissue specificity

    Expressed in the brain and, a lower level, in the heart, skeletal muscle, kidney and placenta. Accumulates in dystrophic neurites around the amyloid core of Alzheimer disease senile plaques (at protein level).
  • Sequence similarities

    Contains 2 cadherin domains.
  • Domain

    The cytoplasmic domain is involved in interaction with APBA2, as well as the binding of synaptic Ca(2+).
  • Post-translational
    modifications

    Proteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by presenilin gamma-secretase within the transmembrane domain releases the beta-Alc-alpha chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection. Cell junction > synapse > postsynaptic cell membrane. Nucleus. Neurite tips. Localized in the postsynaptic membrane of both excitatory and inhibitory synapses (By similarity). The AlcICD fragment is translocated to the nucleus upon interaction with APBB1.
  • Target information above from: UniProt accession O94985 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 22883 Human
    • Entrez Gene: 65945 Mouse
    • Omim: 611321 Human
    • SwissProt: O94985 Human
    • SwissProt: Q9EPL2 Mouse
    • Unigene: 29665 Human
    • Unigene: 38993 Mouse
    • Alternative names

      • Alc alpha antibody
      • Alc-alpha antibody
      • Alcadein alpha 1 antibody
      • Alcadein alpha antibody
      • Alcadein-alpha antibody
      • alcalpha1 antibody
      • alcalpha2 antibody
      • Alzheimer related cadherin like protein antibody
      • Alzheimer-related cadherin-like protein antibody
      • C-terminal fragment 1-alpha antibody
      • Cadherin related family member 12 antibody
      • Calsyntenin 1 antibody
      • Calsyntenin1 antibody
      • CDHR12 antibody
      • CLSTN 1 antibody
      • Clstn1 antibody
      • CS1 antibody
      • CSTN1 antibody
      • CSTN1_HUMAN antibody
      • CTF1-alpha antibody
      • FLJ32258 antibody
      • KIAA0911 antibody
      • Non classical cadherin XB31alpha antibody
      • Non classical cadherin XB31alpha1 antibody
      • Non-classical cadherin XB31alpha antibody
      • PIK3CD antibody
      • SAlc-alpha antibody
      • XB31alpha antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (ab134130)
      Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (ab134130)
      Immfluorescent staining of paraffin embedded Human SH SY 5Y cells labelling CLSTN1 with ab134130 at 1/100 dilution
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (ab134130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (ab134130)

      Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CLSTN1 with ab134130 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
      Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
      All lanes : Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : 293T cell lysate
      Lane 3 : U87 MG lysate
      Lane 4 : SH SY5Y lysate
      Lane 5 : Neuro 2a lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti mouse at 1/2000 dilution

      Predicted band size: 110 kDa

    • Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
      Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
      Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/200000 dilution + Human fetal brain tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 110 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST

      Extra band at: 90kDa   We are unsure as to the identity of this extra band.

    • Anti-CLSTN1 antibody [EPR2963] (ab134130)
      Anti-CLSTN1 antibody [EPR2963] (ab134130)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab134130? Please let us know so that we can cite the reference in this datasheet.

    ab134130 has been referenced in 3 publications.

    • Ou MY  et al. The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development. Cell Rep 35:109290 (2021). PubMed: 34192541
    • Lleó A  et al. Changes in Synaptic Proteins Precede Neurodegeneration Markers in Preclinical Alzheimer's Disease Cerebrospinal Fluid. Mol Cell Proteomics 18:546-560 (2019). PubMed: 30606734
    • Yu L  et al. The Circular RNA Cdr1as Act as an Oncogene in Hepatocellular Carcinoma through Targeting miR-7 Expression. PLoS One 11:e0158347 (2016). PubMed: 27391479

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-CLSTN1 antibody [EPR2963]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (whole brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    whole brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 25 2022

    Western blot abreview for Anti-CLSTN1 antibody [EPR2963]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (RPE1)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    20 µg
    Specification
    RPE1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 02 2020

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