Key features and details
- Rabbit polyclonal to CLUAP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CLUAP1 antibody
See all CLUAP1 primary antibodies
DescriptionRabbit polyclonal to CLUAP1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee
Recombinant fragment within Human CLUAP1 (internal sequence). The exact sequence is proprietary.
Database link: Q96AJ1
- WB: HCT 116 whole cell lysate. ICC/IF: A549 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
FunctionRequired for cilia biogenesis. Appears to function within the multiple intraflagellar transport complex B (IFT-B). Key regulator of hedgehog signaling.
Tissue specificityExpressed in testis, thyroid and trachea and to a lower extent in spinal cord and adrenal gland. Highly expressed in colon cancer and osteosarcoma cell lines.
Sequence similaritiesBelongs to the CLUAP1 family.
Cellular localizationNucleus. Cell projection, cilium.
- Information by UniProt
- CLUA1_HUMAN antibody
- Cluap1 antibody
- Clusterin associated protein 1 antibody
Anti-CLUAP1 antibody (ab227222) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS-PAGE gel.
PFA-fixed A549 (human lung carcinoma cell line) cells stained for CLUAP1 (green) using ab227222 at 1/500 dilution in ICC/IF.
Nuclear Counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227222 has not yet been referenced specifically in any publications.