Recombinant
RabMAb

Recombinant Anti-Clusterin alpha chain antibody [EPR17539-95] - BSA and Azide free (ab230150)

Overview

  • Product name

    Anti-Clusterin alpha chain antibody [EPR17539-95] - BSA and Azide free
    See all Clusterin alpha chain primary antibodies
  • Description

    Rabbit monoclonal [EPR17539-95] to Clusterin alpha chain - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse Clusterin alpha chain aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q06890

  • Positive control

    • IHC-P: Rat adrenal gland tissue.
  • General notes

    ab230150 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab230150 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab230150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681).

IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • Tissue specificity

    Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • Sequence similarities

    Belongs to the clusterin family.
  • Post-translational
    modifications

    Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • Cellular localization

    Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm > cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle > secretory vesicle > chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis and Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40 antibody
    • AAG4 antibody
    • Aging-associated gene 4 protein antibody
    • aging-associated protein 4 antibody
    • Apo-J antibody
    • APOJ antibody
    • ApoJalpha antibody
    • ApoJbeta antibody
    • Apolipoprotein J antibody
    • CLI antibody
    • CLU antibody
    • CLUS_HUMAN antibody
    • Clusterin alpha chain antibody
    • Clusterin antibody
    • Complement cytolysis inhibitor a chain antibody
    • Complement cytolysis inhibitor antibody
    • Complement cytolysis inhibitor b chain antibody
    • complement lysis inhibitor antibody
    • Complement-associated protein SP-40 antibody
    • Complement-associated protein SP-40,40 antibody
    • Ku70-binding protein 1 antibody
    • KUB1 antibody
    • MGC24903 antibody
    • NA1/NA2 antibody
    • SGP-2 antibody
    • SGP2 antibody
    • SP-40,40 antibody
    • sulfated glycoprotein 2 antibody
    • Testosterone-repressed prostate message 2 antibody
    • TRPM-2 antibody
    • TRPM2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184100).

  • Clusterin alpha chain was immunoprecipitated from 0.35 mg mouse serum lysate with ab184100 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184100 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Mouse serum lysate 10 µl (Input).
    Lane 2: ab184100 IP in mouse serum lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184100 in mouse serum lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184100).

  • Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of mouse adrenal gland than the cortex (PMID: 16436671) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184100).

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184100).

  • Immunohistochemical analysis of frozen rat brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in rat brain (PMID:18620027; 21385939) is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184100).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells (PMID: 22552734) is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184100).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in mouse brain (PMID:18620027; 21385939) is observed. 

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184100).

  • Immunohistochemical analysis of 4% paraformaldehude-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells is observed; also observe particles deposited on sperm membrane (PMID: 22552734).

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184100).

  • Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of rat adrenal gland than the cortex (PMID: 16436671) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184100).

     

References

ab230150 has not yet been referenced specifically in any publications.

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