Key features and details
- Rabbit polyclonal to Clusterin
- Suitable for: WB
- Reacts with: Sheep, Goat
- Isotype: IgG
Product nameAnti-Clusterin antibody
See all Clusterin primary antibodies
DescriptionRabbit polyclonal to Clusterin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Sheep, Goat
Predicted to work with: Cow
Recombinant fragment (His-T7-tag) corresponding to Sheep Clusterin aa 20-439. (Expressed in E.coli).
ISDKELQEMSTEGSKYVNKEIKNALKEVKQIKTQIEQTNEERKLLLSSLE EAKKKKEDALNDTRDSENKLKASQGVCNETMTALWEECKPCLKQTCMKFY ARVCRSGSGLVGHQLEEFLNQSSPFYFWINGDRIDSLMENDREQSHVMDV MEDSFTRASSIMDELFQDRFFPRRPQDTQYYSPFSSFPRGSLFFNPKSRF ARNVMPFPLLEPLNFHDVFQPFYDMIRQAQQAMDAHLQRTPYHFPVTEFT ENNDRTVCKEIRHNSTGCLRMKDQCEKCQEILEVDCSASSPTQTLLRQQL NTSLQLAEKFSRLYDQLLQSYQQKMLDTSTLLKQLNEQFTWVSQLANLTQ SDDQYYLQVFTVNSHSSDPSIPSGLTKVVVKLFNSFPITVTVPQEVSSPN FMENVAEKALQQYRRKSHEE
Database link: W5PZI0
- WB: Recombinant sheep Clusterin protein; Goat and sheep testis lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231236 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 52 kDa.|
FunctionIsoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
Tissue specificityDetected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
Sequence similaritiesBelongs to the clusterin family.
modificationsIsoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
Cellular localizationSecreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
- Information by UniProt
- 40 antibody
- AAG 4 antibody
- AAG4 antibody
Anti-Clusterin antibody (ab231236) at 2 µg/ml + Recombinant sheep Clusterin protein
Predicted band size: 52 kDa
Anti-Clusterin antibody (ab231236) at 2 µg/ml + Goat testis lysate
Predicted band size: 52 kDa
Anti-Clusterin antibody (ab231236) at 2 µg/ml + Sheep testis lysate
HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 52 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231236 has not yet been referenced specifically in any publications.