Anti-Clusterin antibody (ab79280)
Key features and details
- Goat polyclonal to Clusterin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Clusterin antibody
See all Clusterin primary antibodies -
Description
Goat polyclonal to Clusterin -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Mouse Clusterin aa 435-448 (C terminal).
Sequence:C-EKALQEYRRKSRAE
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab79280 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab79280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB |
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. -
Tissue specificity
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. -
Sequence similarities
Belongs to the clusterin family. -
Post-translational
modificationsIsoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. -
Cellular localization
Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 12759 Mouse
- Entrez Gene: 24854 Rat
- SwissProt: Q06890 Mouse
- SwissProt: P05371 Rat
- Unigene: 200608 Mouse
- Unigene: 1780 Rat
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Alternative names
- 40 antibody
- AAG 4 antibody
- AAG4 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Clusterin antibody (ab79280)
HeLa cells stained for Clusterin (green) using ab79280 at 10 µg/ml in ICC/IF. Cells were permeabilized with 0.15% Triton. DAPI was used as a nuclear counterstain.
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Western blot - Anti-Clusterin antibody (ab79280)Anti-Clusterin antibody (ab79280) at 0.3 µg/ml + Human Testis lysate at 35 µg
Predicted band size: 52 kDa -
Western blot - Anti-Clusterin antibody (ab79280)Anti-Clusterin antibody (ab79280) at 0.3 µg/ml + Rat Brain lysate (in RIPA buffer) at 35 µg
Predicted band size: 52 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18;104(12):1407-12.; PMID: 11560857).
Datasheets and documents
References (0)
ab79280 has not yet been referenced specifically in any publications.
