Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain lysate)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
Brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1M TBST with 1%milk
Dilution
1/500
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: DAKO anti rabbit
Host species: Pig
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 10 kDa
Positive control
Normal mouse liver
Additional data
Additional Notes
Western Blot: 4-12% Bis-Tris gel probed with Rabbit monoclonal Apo J (Ab92548) antibody. Lane 1: 3 months old mouse liver, lane 2: Lane: 12 months old control brain, Lane 3: 12month old APP-tg brain, (antibody dilution 1 in 200)
DR. Ruma Raha-Chowdhury
Verified customer
Submitted Feb 05 2016