For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    clusterin-antibody-epr2911-bsa-and-azide-free-ab271867.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Complement Classical Pathway
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
  • Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
  • Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
  • Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2911] to Clusterin - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Primary
Product image
Anti-CD164 antibody (ab238748)

View more associated products

Overview

  • Product name

    Anti-Clusterin antibody [EPR2911] - BSA and Azide free
    See all Clusterin primary antibodies
  • Description

    Rabbit monoclonal [EPR2911] to Clusterin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human breast carcinoma and testis tissue.
  • General notes

    ab271867 is the carrier-free version of ab92548.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2911
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • Anti-Clusterin antibody [EPR2911] (ab92548)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

 

WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
Notes
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

 

WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.

Target

  • Function

    Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • Tissue specificity

    Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • Sequence similarities

    Belongs to the clusterin family.
  • Post-translational
    modifications

    Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • Cellular localization

    Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
  • Target information above from: UniProt accession P10909 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1191 Human
    • Omim: 185430 Human
    • SwissProt: P10909 Human
    • Unigene: 436657 Human
    • Alternative names

      • 40 antibody
      • AAG 4 antibody
      • AAG4 antibody
      • Aging associated protein 4 antibody
      • Aging-associated gene 4 protein antibody
      • AI893575 antibody
      • APO J antibody
      • Apo-J antibody
      • APOJ antibody
      • ApoJalpha antibody
      • ApoJbeta antibody
      • Apolipoprotein J antibody
      • ApolipoproteinJ antibody
      • CLI antibody
      • CLU antibody
      • CLU1 antibody
      • CLU2 antibody
      • CLUS_HUMAN antibody
      • Clusterin alpha chain antibody
      • Clusterin antibody
      • Clusterin beta chain antibody
      • Complement associated protein SP 40 40 antibody
      • Complement associated protein SP 40 antibody
      • Complement associated protein SP40 antibody
      • Complement cytolysis inhibitor a chain antibody
      • Complement cytolysis inhibitor antibody
      • Complement cytolysis inhibitor b chain antibody
      • Complement lysis inhibitor antibody
      • Complement-associated protein SP-40 antibody
      • D14Ucla3 antibody
      • Dimeric acid glycoprotein antibody
      • Glycoprotein 80 antibody
      • Glycoprotein III antibody
      • GP80 antibody
      • Ku70-binding protein 1 antibody
      • KUB 1 antibody
      • KUB1 antibody
      • MGC24903 antibody
      • NA1/NA2 antibody
      • RATTRPM2B antibody
      • SGP 2 antibody
      • SGP2 antibody
      • SP 40 antibody
      • SP40 antibody
      • Sugp-2 antibody
      • Sulfated glycoprotein 2 antibody
      • Testosterone repressed prostate message 2 antibody
      • Testosterone-repressed prostate message 2 antibody
      • TRPM 2 antibody
      • TRPM-2 antibody
      • TRPM2 antibody
      • TRPM2B antibody
      • Trpmb antibody
      see all

    Images

    • Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
      Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
      All lanes : Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution

      Lane 1 : Human testis lysates
      Lane 2 : Human brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 37,68 kDa why is the actual band size different from the predicted?



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548).

      Clusterin precursor could be cleaved to produce an N-terminal α-chain and a C-terminal β-chain as was described by PMID: 25402950. ab92548 recognizes α-chain.

    • Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
      Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
      All lanes : Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution

      Lane 1 : Human tonsil tissue lysate
      Lane 2 : Fetal brain tissue lysate
      Lane 3 : Human Plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548).

    • Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
      Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548).

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Clusterin knockout HAP1 whole cell lysate (20 µg)
      Lane 3: U87-MG whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab92548 observed at 68 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab92548 was shown to recognize Clusterin in wild-type HAP1 cells as signal was lost at the expected MW in Clusterin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Clusterin knockout samples were subjected to SDS-PAGE. Ab92548 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Clusterin with purified ab92548 at 1/450 dilution (0.23 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)

      ab92548 (unpurified) at 1/100 dilution staining Apolipoprotein J in Human testis tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548).
    • Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
      Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab271867? Please let us know so that we can cite the reference in this datasheet.

    ab271867 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Anti-Clusterin antibody [EPR2911] - BSA and Azide free

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Immortalized Human Astrocytes)
    Permeabilization
    Yes - CyTOF permeabilization buffer
    Specification
    Immortalized Human Astrocytes
    Preparation
    Cell harvesting/tissue preparation method: Trypsin-EDTA for 2-3 minutes
    Sample buffer: PriGrow 4
    Fixation
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 22 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.