Recombinant Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
![Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)](https://www.abcam.com/ps/products/271/ab271867/Images/ab271867-483313-ab92548351357anticlusterinantibodyepr2911westernblothuman.jpg "Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2911] to Clusterin - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Clusterin antibody [EPR2911] - BSA and Azide free
See all Clusterin primary antibodies -
Description
Rabbit monoclonal [EPR2911] to Clusterin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: human breast carcinoma and testis tissue.
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General notes
ab271867 is the carrier-free version of ab92548.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2911 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
See IHC antigen retrieval protocols.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
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| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. See IHC antigen retrieval protocols.
|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. |
Target
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Function
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. -
Tissue specificity
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. -
Sequence similarities
Belongs to the clusterin family. -
Post-translational
modificationsIsoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. -
Cellular localization
Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 1191 Human
- Omim: 185430 Human
- SwissProt: P10909 Human
- Unigene: 436657 Human
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Alternative names
- 40 antibody
- AAG 4 antibody
- AAG4 antibody
see all
Images
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Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)All lanes : Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution
Lane 1 : Human testis lysates
Lane 2 : Human brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 37,68 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548).
Clusterin precursor could be cleaved to produce an N-terminal α-chain and a C-terminal β-chain as was described by PMID: 25402950. ab92548 recognizes α-chain.
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![Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)](https://www.abcam.com/ps/products/271/ab271867/Images/ab271867-483312-ab92548107700anticlusterinantibodyepr2911westernblot.jpg)
Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)All lanes : Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution
Lane 1 : Human tonsil tissue lysate
Lane 2 : Fetal brain tissue lysate
Lane 3 : Human Plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548).
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![Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)](https://www.abcam.com/ps/products/271/ab271867/Images/ab271867-483311-ab92548297744anticlusterinantibodyepr2911westernblot.jpg)
Western blot - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548).
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Clusterin knockout HAP1 whole cell lysate (20 µg)
Lane 3: U87-MG whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab92548 observed at 68 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab92548 was shown to recognize Clusterin in wild-type HAP1 cells as signal was lost at the expected MW in Clusterin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Clusterin knockout samples were subjected to SDS-PAGE. Ab92548 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)](https://www.abcam.com/ps/products/271/ab271867/Images/ab271867-3-ab925485anticlusterinantibodyepr2911immunohistochemistrybreastcarcinomahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Clusterin with purified ab92548 at 1/450 dilution (0.23 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)](https://www.abcam.com/ps/products/271/ab271867/Images/ab271867-1-ab92548107699anticlusterinantibodyepr2911immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)ab92548 (unpurified) at 1/100 dilution staining Apolipoprotein J in Human testis tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92548). -
![Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)](https://www.abcam.com/ps/products/271/ab271867/Images/ab271867-4-benefits-of-recombinant-antibodies.png)
Anti-Clusterin antibody [EPR2911] - BSA and Azide free (ab271867)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab271867 has not yet been referenced specifically in any publications.
