Product nameAnti-CMAS antibody [EPR10485]
DescriptionRabbit monoclonal [EPR10485] to CMAS
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human CMAS (UniProt: Q8NFW8).
- RAW264.7, Human fetal brain, HeLa and 293T lysates; 293 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab157189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 48 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN).
Tissue specificityUbiquitously expressed. Expressed in pancreas, kidney, liver, skeletal muscle, lung, placenta, brain, heart, colon, PBL, small intestine, ovary, testis, prostate, thymus and spleen.
PathwayAmino-sugar metabolism; N-acetylneuraminate metabolism.
Sequence similaritiesBelongs to the CMP-NeuNAc synthase family.
DomainThe BC2 (basic cluster 2) motif is necessary and sufficient for the nuclear localization and contains the catalytic active site. The localization in the nucleus is however not required for the enzyme activity.
- Information by UniProt
- CMAS antibody
- CMP N acetylneuraminic acid synthase antibody
- CMP Neu5Ac synthetase antibody
All lanes : Anti-CMAS antibody [EPR10485] (ab157189) at 1/1000 dilution
Lane 1 : RAW264.7 cell lysate
Lane 2 : Human fetal brain lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDa
Flow cytometric analysis of 293 cells labeling CMAS with ab157189 at 1/100 (red) or a rabbit IgG (negative) (green).
ab157189 has not yet been referenced specifically in any publications.