Key features and details
- Rabbit polyclonal to CMIP
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CMIP antibody
See all CMIP primary antibodies
DescriptionRabbit polyclonal to CMIP
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Xenopus laevis
Synthetic peptide within Human CMIP aa 723-773. The exact sequence is proprietary.
Database link: Q8IY22
- IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241165 was affinity purified using an epitope specific to CMIP immobilized on solid support.
Our Abpromise guarantee covers the use of ab241165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlays a role in T-cell signaling pathway. Isoform 2 may play a role in T-helper 2 (Th2) signaling pathway and seems to represent the first proximal signaling protein that links T-cell receptor-mediated signal to the activation of c-Maf Th2 specific factor.
Tissue specificityIsoform 1 is expressed in peripheral blood mononuclear cells and kidney. Lower expression in brain and liver. Expression is down-regulated in activated cells. Isoform 2 is expressed in lymphocyte precursors, however, expression shuts down during maturation and differentiation in thymus and fetal liver.
Sequence similaritiesContains 4 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Developmental stageExpressed in fetal liver.
Cellular localizationNucleus. Cytoplasm. Isoform 2 is translocated to the nucleus and is specifically recruited during minimal change nephrotic syndrome (MCNS). Detected in nuclear and cytoplasmic compartments during MCNS relapse. Expressed in cytoplasm only during MCNS remission and absent in normal patients.
- Information by UniProt
- C-Maf-inducing protein antibody
- c-Mip antibody
- cmip antibody
CMIP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241165 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241165 at 1 µg/ml.
Lane 1: ab241165 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 75 seconds.
Lysate prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241165 has not yet been referenced specifically in any publications.