Key features and details
- Rabbit polyclonal to CMKLR1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CMKLR1 antibody
See all CMKLR1 primary antibodies
DescriptionRabbit polyclonal to CMKLR1
Specificityab230442 recognizes endogenous levels of CMKLR1 protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Pig
Synthetic peptide corresponding to Human CMKLR1 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q99788
- WB: Jurkat and HepG2 whole cell lysate (ab7900); Mouse liver whole cell cell lysate; rat liver whole cell lysate. IHC-P: Human mesothelium and breast cancer tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionOrphan receptor. Could be a chemotactic peptide receptor. May have a function in bone metabolism. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).
Tissue specificityProminently expressed in developing osseous and cartilaginous tissue. Also found in adult parathyroid glands.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Developmental stageExpressed during bone development.
Cellular localizationCell membrane.
- Information by UniProt
- CHEMERINR antibody
- Chemokine like receptor 1 antibody
- Chemokine-like receptor 1 antibody
All lanes : Anti-CMKLR1 antibody (ab230442) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Mouse liver whole cell lysate
Lane 4 : Rat liver whole cell lysate
Developed using the ECL technique.
Predicted band size: 42 kDa
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for CMKLR1 with ab230442 at 1/100 dilution in immunohistochemical analysis. Incubated with the primary antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Formalin-fixed, paraffin-embedded human mesothelium tissue stained for CMKLR1 with ab230442 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230442 has been referenced in 1 publication.
- Smolinska N et al. Expression of Chemerin and Its Receptors in the Porcine Hypothalamus and Plasma Chemerin Levels during the Oestrous Cycle and Early Pregnancy. Int J Mol Sci 20:N/A (2019). PubMed: 31404950