RecombinantRabMAb

Recombinant Anti-CMT2 antibody [EPR9584] (ab150363)

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Western blot - Anti-CMT2 antibody [EPR9584] (ab150363)
  • Immunocytochemistry/ Immunofluorescence - Anti-CMT2 antibody [EPR9584] (ab150363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] (ab150363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] (ab150363)
  • Anti-CMT2 antibody [EPR9584] (ab150363)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9584] to CMT2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CMT2 antibody [EPR9584]
    See all CMT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9584] to CMT2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human CMT2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15013

  • Positive control

    • Recombinant Human CMT2 (ab123183) can be used as a positive control in WB. HepG2, A431, and HeLa cell lysates, Human brain and Human cervical carcinoma tissues This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

  • General notes

     

     

     This product was previously labelled as MAD2L1 binding protein

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9584

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.

    • Sequence similarities

      Belongs to the MAD2L1BP family.

    • Developmental stage

      During the cell cycle, levels increase and then remain constant until late mitosis after which they drop.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
    • Information by UniProt

    • Database links

      • Alternative names

        • Caught by MAD2 protein antibody
        • CMT2 antibody
        • dJ261G23.1 antibody
        • KIAA0110 antibody
        • MAD2L1 binding protein antibody
        • MAD2L1 binding protein isoform 2 antibody
        • MAD2L1-binding protein antibody
        • Mad2l1bp antibody
        • MD2BP_HUMAN antibody
        • MGC11282 antibody
        • OTTHUMP00000016496 antibody
        • RP1 261G23.6 antibody
        see all

      Images

      • Western blot - Anti-CMT2 antibody [EPR9584] (ab150363)
        Western blot - Anti-CMT2 antibody [EPR9584] (ab150363)
        All lanes : Anti-CMT2 antibody [EPR9584] (ab150363) at 1/1000 dilution

        Lane 1 : HepG2 cell lysate
        Lane 2 : A431 cell lysate
        Lane 3 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 31 kDa
        Observed band size: 34 kDa why is the actual band size different from the predicted?

      • Immunocytochemistry/ Immunofluorescence - Anti-CMT2 antibody [EPR9584] (ab150363)
        Immunocytochemistry/ Immunofluorescence - Anti-CMT2 antibody [EPR9584] (ab150363)

        ab150363 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab150363 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] (ab150363)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] (ab150363)

        Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CMT2 binding protein with ab150363 antibody at a dilution of 1/100.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] (ab150363)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] (ab150363)

        Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labelling CMT2 binding protein with ab150363 antibody at a dilution of 1/100.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-CMT2 antibody [EPR9584] (ab150363)
        Anti-CMT2 antibody [EPR9584] (ab150363)

      References (3)

      Publishing research using ab150363? Please let us know so that we can cite the reference in this datasheet.

      ab150363 has been referenced in 3 publications.

      • Shin HJ  et al. p31comet-Induced Cell Death Is Mediated by Binding and Inactivation of Mad2. PLoS One 10:e0141523 (2015). WB . PubMed: 26544187
      • de Lange J  et al. Defective sister chromatid cohesion is synthetically lethal with impaired APC/C function. Nat Commun 6:8399 (2015). WB . PubMed: 26423134
      • Date DA  et al. Phosphorylation regulates the p31Comet-mitotic arrest-deficient 2 (Mad2) interaction to promote spindle assembly checkpoint (SAC) activity. J Biol Chem 289:11367-73 (2014). PubMed: 24596092

      Customer reviews and Q&As

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      Western blot abreview for Anti-MAD2L1 binding protein antibody [EPR9584]

       Below Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (hRPE1)
      Gel Running Conditions
      Reduced Denaturing (NOVEX 4-12% A/a)
      Loading amount
      60 µg
      Specification
      hRPE1
      Blocking step
      Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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      Submitted Dec 17 2018

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