RecombinantRabMAb

Recombinant Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)

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Western blot - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
  • Immunocytochemistry - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
  • Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9584] to CMT2 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CMT2 antibody [EPR9584] - BSA and Azide free
    See all CMT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9584] to CMT2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248941 is the carrier-free version of ab150363 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248941 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as MAD2L1 binding protein

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9584

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.

    • Sequence similarities

      Belongs to the MAD2L1BP family.

    • Developmental stage

      During the cell cycle, levels increase and then remain constant until late mitosis after which they drop.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
    • Information by UniProt

    • Database links

      • Alternative names

        • Caught by MAD2 protein antibody
        • CMT2 antibody
        • dJ261G23.1 antibody
        • KIAA0110 antibody
        • MAD2L1 binding protein antibody
        • MAD2L1 binding protein isoform 2 antibody
        • MAD2L1-binding protein antibody
        • Mad2l1bp antibody
        • MD2BP_HUMAN antibody
        • MGC11282 antibody
        • OTTHUMP00000016496 antibody
        • RP1 261G23.6 antibody
        see all

      Images

      • Western blot - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
        Western blot - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
        All lanes : Anti-CMT2 antibody [EPR9584] (ab150363) at 1/1000 dilution

        Lane 1 : HepG2 cell lysate
        Lane 2 : A431 cell lysate
        Lane 3 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 31 kDa
        Observed band size: 34 kDa why is the actual band size different from the predicted?



        This data was developed using ab150363, the same antibody clone in a different buffer formulation.

      • Immunocytochemistry - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
        Immunocytochemistry - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
        This data was developed using ab150363, the same antibody clone in a different buffer formulation.ab150363 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab150363 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
        This data was developed using ab150363, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CMT2 binding protein with ab150363 antibody at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
        This data was developed using ab150363, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labelling CMT2 binding protein with ab150363 antibody at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
        Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab248941? Please let us know so that we can cite the reference in this datasheet.

      ab248941 has not yet been referenced specifically in any publications.

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