Recombinant Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-CMT2 antibody [EPR9584] - BSA and Azide free
See all CMT2 primary antibodies -
Description
Rabbit monoclonal [EPR9584] to CMT2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CMT2 aa 250-350. The exact sequence is proprietary.
Database link: Q15013 -
General notes
ab248941 is the carrier-free version of ab150363 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248941 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as MAD2L1 binding protein
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9584 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab248941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa). |
Target
-
Function
May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. -
Sequence similarities
Belongs to the MAD2L1BP family. -
Developmental stage
During the cell cycle, levels increase and then remain constant until late mitosis after which they drop. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle. - Information by UniProt
-
Database links
- Entrez Gene: 9587 Human
- Entrez Gene: 66591 Mouse
- Entrez Gene: 316237 Rat
- SwissProt: Q15013 Human
- SwissProt: Q9DCX1 Mouse
- Unigene: 122346 Human
- Unigene: 715485 Human
- Unigene: 272024 Mouse
-
Alternative names
- Caught by MAD2 protein antibody
- CMT2 antibody
- dJ261G23.1 antibody
see all
Datasheets and documents
Certificate of Compliance
References
ab248941 has not yet been referenced specifically in any publications.
