Anti-CMTM5 antibody - N-terminal (ab187980)
Key features and details
- Rabbit polyclonal to CMTM5 - N-terminal
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CMTM5 antibody - N-terminal -
Description
Rabbit polyclonal to CMTM5 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CMTM5 aa 1-100 (N terminal).
Database link: Q96DZ9 -
Positive control
- IHC-P: Human cerebral cortex, pancreas tissue; WB: Transfected HEK-293T and HEL whole cell lysates; ICC: HEL whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa. |
Target
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Tissue specificity
Highly expressed in the brain. -
Sequence similarities
Belongs to the chemokine-like factor family.
Contains 1 MARVEL domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 116173 Human
- Omim: 607888 Human
- SwissProt: Q96DZ9 Human
- Unigene: 99272 Human
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Alternative names
- 1500005P16Rik antibody
- 2900052H21Rik antibody
- Chemokine like factor super family 5 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling CMTM5 (high expression) with ab187980 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-CMTM5 antibody - N-terminal (ab187980) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : CMTM5 over-expression - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 25 kDa -
Immunocytochemical analysis of HEL (Human bone marrow erythroleukemia cell line) whole cells labeling CMTM5 in the Golgi apparatus and vesicles with ab187980 at 2 µg/ml.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CMTM5 (low expression) with ab187980 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-CMTM5 antibody - N-terminal (ab187980) at 0.4 µg/ml + HEL (Human bone marrow erythroleukemia cell line) whole cell lysate
Predicted band size: 25 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab187980 has been referenced in 2 publications.
- Li L et al. CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway. Mol Med Rep 25:N/A (2022). PubMed: 34791506
- Xu G & Dang C CMTM5 is downregulated and suppresses tumour growth in hepatocellular carcinoma through regulating PI3K-AKT signalling. Cancer Cell Int 17:113 (2017). PubMed: 29213215