Recombinant Anti-CMTM6 antibody [EPR23015-45] (ab264067)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23015-45] to CMTM6
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CMTM6 antibody [EPR23015-45]
See all CMTM6 primary antibodies -
Description
Rabbit monoclonal [EPR23015-45] to CMTM6 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MDA-MB-231, Raji and BxPC-3 whole cell lysates; human tonsil tissue lysate. IHC-P: Human tonsil, thyroid cancer and pancreatic cancer tissue. Flow Cyt (intra): MDA-MB-231 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23015-45 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/500.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 20 kDa.
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Notes |
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Flow Cyt (Intra)
1/500. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 20 kDa. |
Target
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Tissue specificity
Expressed in the leukocytes, placenta and testis. -
Sequence similarities
Belongs to the chemokine-like factor family.
Contains 1 MARVEL domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 54918 Human
- Omim: 607889 Human
- SwissProt: Q9NX76 Human
- Unigene: 380627 Human
- Unigene: 440494 Human
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Alternative names
- chemokine like factor super family 6 antibody
- chemokine like factor superfamily 6 antibody
- chemokine like factor superfamily member 6 antibody
see all
Images
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All lanes : Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/10000 dilution
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate boiled
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate unboiled
Lane 3 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate boiled
Lane 4 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate unboiled
Lane 5 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate boiled
Lane 6 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 7 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
ab181602 was used as GAPDH loading control.
We recommend not to boil the samples after lysis to get desired WB bands.
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All lanes : Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/1000 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Human tonsil tissue lysate
Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1-2: 3 minutes
Lane 3: 48 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28813417).
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Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human pancreatic cancer (PMID/ 28813417) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human thyroid cancer (PMID: 28813410) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling CMTM6 with ab264067 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730)isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab264067 has been referenced in 2 publications.
- Shi S et al. Expression and Clinical Significance of CMTM6 and PD-L1 in Triple-Negative Breast Cancer. Biomed Res Int 2022:8118909 (2022). PubMed: 35845949
- Liu Z et al. Computational Recognition and Clinical Verification of TGF-β-Derived miRNA Signature With Potential Implications in Prognosis and Immunotherapy of Intrahepatic Cholangiocarcinoma. Front Oncol 11:757919 (2021). PubMed: 34760703