Anti-CNBP antibody (ab48027)
Key features and details
- Goat polyclonal to CNBP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CNBP antibody
See all CNBP primary antibodies -
Description
Goat polyclonal to CNBP -
Host species
Goat -
Specificity
No crossreaction expected with CCHC domain containing 13. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Macaque monkey
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Immunogen
Synthetic peptide:
GESGHLARECTIE
, corresponding to C terminal amino acids 162-174 of Human CNBP. -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab48027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
WB: Use at a concentration of 1-3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression. -
Tissue specificity
Present in all tissues examined. -
Involvement in disease
Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene. -
Sequence similarities
Contains 7 CCHC-type zinc fingers. -
Cellular localization
Cytoplasm. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 395731 Chicken
- Entrez Gene: 504831 Cow
- Entrez Gene: 7555 Human
- Entrez Gene: 12785 Mouse
- Entrez Gene: 399294 Xenopus laevis
- Omim: 116955 Human
- SwissProt: O42395 Chicken
- SwissProt: Q3T0Q6 Cow
see all -
Alternative names
- CCHC type zinc finger nucleic acid binding protein antibody
- Cellular nucleic acid binding protein antibody
- Cellular nucleic acid-binding protein antibody
see all
Images
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Western blot - Anti-CNBP antibody (ab48027)Anti-CNBP antibody (ab48027) at 2 µg/ml + 35µg Human muscle lysate in RIPA buffer
Secondary
anti-goat-HRP at a 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 19 kDa -
Western blot - Anti-CNBP antibody (ab48027)Image courtesy of an anonymous Abreview.All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutes
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Western blot - Anti-CNBP antibody (ab48027)Image courtesy of an anonymous Abreview.All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells
Lane 2 : Whole cell lysate prepared from F9 cells
Lane 3 : Whole cell lysate prepared from GC-1 cells
Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Datasheets and documents
References (2)
ab48027 has been referenced in 2 publications.
- Yang F et al. Circ-HuR suppresses HuR expression and gastric cancer progression by inhibiting CNBP transactivation. Mol Cancer 18:158 (2019). PubMed: 31718709
- Antonucci L et al. CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMyc. Cell Cycle 13:434-9 (2014). PubMed: 24275942
