For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cnbp-antibody-ab48027.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Other
Share by email

Anti-CNBP antibody (ab48027)

  • Datasheet
Reviews (3)Q&A (1)References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-CNBP antibody (ab48027)
  • Western blot - Anti-CNBP antibody (ab48027)
  • Western blot - Anti-CNBP antibody (ab48027)

Key features and details

  • Goat polyclonal to CNBP
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Donkey Anti-Goat IgG H&L (HRP) (ab97110)

View more associated products

Overview

  • Product name

    Anti-CNBP antibody
    See all CNBP primary antibodies
  • Description

    Goat polyclonal to CNBP
  • Host species

    Goat
  • Specificity

    No crossreaction expected with CCHC domain containing 13.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Macaque monkey
  • Immunogen

    Synthetic peptide:

    GESGHLARECTIE

    , corresponding to C terminal amino acids 162-174 of Human CNBP.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human CNBP protein (ab140548)

Applications

Our Abpromise guarantee covers the use of ab48027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    ELISA: 1/8000 (detection limit).
    WB: Use at a concentration of 1-3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.
    • Tissue specificity

      Present in all tissues examined.
    • Involvement in disease

      Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene.
    • Sequence similarities

      Contains 7 CCHC-type zinc fingers.
    • Cellular localization

      Cytoplasm. Endoplasmic reticulum.
    • Target information above from: UniProt accession P62633 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 395731 Chicken
      • Entrez Gene: 504831 Cow
      • Entrez Gene: 7555 Human
      • Entrez Gene: 12785 Mouse
      • Entrez Gene: 399294 Xenopus laevis
      • Omim: 116955 Human
      • SwissProt: O42395 Chicken
      • SwissProt: Q3T0Q6 Cow
      • SwissProt: P62633 Human
      • SwissProt: P53996 Mouse
      • Unigene: 518249 Human
      • Unigene: 290251 Mouse
      see all
    • Alternative names

      • CCHC type zinc finger nucleic acid binding protein antibody
      • Cellular nucleic acid binding protein antibody
      • Cellular nucleic acid-binding protein antibody
      • CNBP antibody
      • CNBP_HUMAN antibody
      • CNBP1 antibody
      • DM2 antibody
      • Erythroid differentiation related antibody
      • PROMM antibody
      • Proximal myotonic myopathy nucleic acid binding protein antibody
      • RNF163 antibody
      • Sterol regulatory element binding protein antibody
      • ZCCHC22 antibody
      • Zinc finger protein 273 antibody
      • Zinc finger protein 9 (a cellular retroviral nucleic acid binding protein) antibody
      • Zinc finger protein 9 antibody
      • ZNF9 antibody
      see all

    Images

    • Western blot - Anti-CNBP antibody (ab48027)
      Western blot - Anti-CNBP antibody (ab48027)
      Anti-CNBP antibody (ab48027) at 2 µg/ml + 35µg Human muscle lysate in RIPA buffer

      Secondary
      anti-goat-HRP at a 1/3000 dilution

      Developed using the ECL technique.

      Predicted band size: 19 kDa
      Observed band size: 19 kDa

    • Western blot - Anti-CNBP antibody (ab48027)
      Western blot - Anti-CNBP antibody (ab48027)Image courtesy of an anonymous Abreview.
      All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml

      Lane 1 : Whole cell lysate prepared from 9L cells
      Lane 2 : Whole cell lysate prepared from PC12 cells
      Lane 3 : Whole cell lysate prepared from RIE-1 cells

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution

      Developed using the ECL technique.

      Predicted band size: 19 kDa
      Observed band size: 20 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 6 minutes

      See Abreview

    • Western blot - Anti-CNBP antibody (ab48027)
      Western blot - Anti-CNBP antibody (ab48027)Image courtesy of an anonymous Abreview.
      All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml

      Lane 1 : Whole cell lysate prepared from MEF cells
      Lane 2 : Whole cell lysate prepared from F9 cells
      Lane 3 : Whole cell lysate prepared from GC-1 cells
      Lane 4 : Whole cell lysate prepared from NIH-3T3 cells

      Lysates/proteins at 20 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 19 kDa
      Observed band size: 20 kDa why is the actual band size different from the predicted?
      Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes

      See Abreview

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab48027? Please let us know so that we can cite the reference in this datasheet.

    ab48027 has been referenced in 2 publications.

    • Yang F  et al. Circ-HuR suppresses HuR expression and gastric cancer progression by inhibiting CNBP transactivation. Mol Cancer 18:158 (2019). PubMed: 31718709
    • Antonucci L  et al. CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMyc. Cell Cycle 13:434-9 (2014). PubMed: 24275942

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-CNBP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse cell lines)
    Loading amount
    20 µg
    Specification
    Mouse cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 23 2012

    Western blot abreview for Anti-CNBP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat cell lines)
    Loading amount
    20 µg
    Specification
    Rat cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 22 2012

    Question

    ab83038 and ab48027: Both of these antibodies are aganist CNBP. Your data sheet o each sayu that each antibody has been tried and works in WB abd ELISA. Other applications have not been tried. However, your spec sheets say both give antigen localization to the endoplasmic reticulum and the cytoplasm, suggesting immunoflorescence has been done with these antibodies. I am interested in using these antibodies for immunoflorescence. Have one, both or neither been used for Immunoflorescence? If so, please let me know. If you have any parameters for IF from in house or a customer on either of these antibodies that would be a great help.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 27 2011

    Answer

    Thank you very much for your interest in our anti-CNBP antibodies. To our knowledge, neither ab48027 nor ab83038 have been tested in cell staining (immunocytochemistry, ICC/IF). The information about localization in the ER and cytoplasm comes from the SwissProt entry for the protein regarding where the protein is known to localize, not where it has been imaged by us, per se: http://www.uniprot.org/uniprot/P62633 Since neither of these antibodies have been tested in ICC/IF, I can offer a discount off a future purchase if you buy ab48027 or ab83038 now, test it in ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab48027 or ab83038 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab48027 or ab83038 so please wait for my reply before ordering. 2. Purchase ab48027 or ab83038 either by phone, fax, or online (www.abcam.com). 3. Test it in ICC/IF. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab48027 or ab83038 turn out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. If you would like to choose 1 of these antibodies to test, let me know which and I will set you up in our program. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Sep 27 2011

    Western blot abreview for Anti-CNBP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T HeLa U251 Jurkat U2OS)
    Loading amount
    20 µg
    Specification
    293T HeLa U251 Jurkat U2OS
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 01 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.