Anti-CNBP antibody (ab48027)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-CNBP antibody
See all CNBP primary antibodies -
DescriptionGoat polyclonal to CNBP
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Host speciesGoat
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SpecificityNo crossreaction expected with CCHC domain containing 13.
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Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Macaque monkey
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Immunogen
Synthetic peptide:
GESGHLARECTIE
, corresponding to C terminal amino acids 162-174 of Human CNBP. -
Positive control
- Human muscle lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab48027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| ELISA | ||
| WB |
Application notes
ELISA: 1/8000 (detection limit).
WB: Use at a concentration of 1-3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1-3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionSingle stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.
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Tissue specificityPresent in all tissues examined.
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Involvement in diseaseDefects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene.
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Sequence similaritiesContains 7 CCHC-type zinc fingers.
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Cellular localizationCytoplasm. Endoplasmic reticulum.
- Information by UniProt
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Database links
- Entrez Gene: 395731 Chicken
- Entrez Gene: 504831 Cow
- Entrez Gene: 7555 Human
- Entrez Gene: 12785 Mouse
- Entrez Gene: 64530 Rat
- Entrez Gene: 399294 Xenopus laevis
- Omim: 116955 Human
- SwissProt: O42395 Chicken
see all -
Alternative names
- CCHC type zinc finger nucleic acid binding protein antibody
- Cellular nucleic acid binding protein antibody
- Cellular nucleic acid-binding protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab48027)ICC/IF image of ab48027 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab48027 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-CNBP antibody (ab48027)Anti-CNBP antibody (ab48027) at 2 µg/ml + 35µg Human muscle lysate in RIPA buffer
Secondary
anti-goat-HRP at a 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 19 kDa -
Western blot - Anti-CNBP antibody (ab48027)Image courtesy of an anonymous Abreview.All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutes
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Western blot - Anti-CNBP antibody (ab48027)Image courtesy of an anonymous Abreview.All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells
Lane 2 : Whole cell lysate prepared from F9 cells
Lane 3 : Whole cell lysate prepared from GC-1 cells
Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Datasheets and documents
References
This product has been referenced in:
- Antonucci L et al. CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMyc. Cell Cycle 13:434-9 (2014). Read more (PubMed: 24275942) »
