Anti-CNDP2/CN2 antibody (ab241126)
Key features and details
- Rabbit polyclonal to CNDP2/CN2
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CNDP2/CN2 antibody
See all CNDP2/CN2 primary antibodies -
Description
Rabbit polyclonal to CNDP2/CN2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan
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Immunogen
Synthetic peptide within Human CNDP2/CN2 aa 100-150. The exact sequence is proprietary.
Database link: Q96KP4 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
This product was previously labelled as CNDP2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241126 was affinity purified using an epitope specific to CNDP2/CN2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 53 kDa. |
Target
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Function
Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Isoform 2 may be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. -
Tissue specificity
Isoform 1 is ubiquitously expressed with higher levels in kidney and liver (at protein level). Isoform 2 is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. -
Sequence similarities
Belongs to the peptidase M20A family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 512626 Cow
- Entrez Gene: 55748 Human
- Entrez Gene: 66054 Mouse
- Entrez Gene: 100174694 Orangutan
- Entrez Gene: 291394 Rat
- Omim: 169800 Human
- SwissProt: Q3ZC84 Cow
- SwissProt: Q96KP4 Human
see all -
Alternative names
- 0610010E05Rik antibody
- C76600 antibody
- Carnosinase , tissue antibody
see all
Images
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Western blot - Anti-CNDP2/CN2 antibody (ab241126)All lanes : Anti-CNDP2/CN2 antibody (ab241126) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 30 secondsLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-CNDP2/CN2 antibody (ab241126)CNDP2/CN2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241126 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241126 at 1 µg/ml.
Lane 1: ab241126 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared using NETN buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241126 has not yet been referenced specifically in any publications.
