Anti-CNDP2/CN2 antibody (ab241126)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CNDP2/CN2 antibody
See all CNDP2/CN2 primary antibodies -
Description
Rabbit polyclonal to CNDP2/CN2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan
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Immunogen
Synthetic peptide within Human CNDP2/CN2 aa 100-150. The exact sequence is proprietary.
Database link: Q96KP4 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
This product was previously labelled as CNDP2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241126 was affinity purified using an epitope specific to CNDP2/CN2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 53 kDa. |
Target
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Function
Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Isoform 2 may be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. -
Tissue specificity
Isoform 1 is ubiquitously expressed with higher levels in kidney and liver (at protein level). Isoform 2 is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. -
Sequence similarities
Belongs to the peptidase M20A family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 512626 Cow
- Entrez Gene: 55748 Human
- Entrez Gene: 66054 Mouse
- Entrez Gene: 100174694 Orangutan
- Entrez Gene: 291394 Rat
- Omim: 169800 Human
- SwissProt: Q3ZC84 Cow
- SwissProt: Q96KP4 Human
see all -
Alternative names
- 0610010E05Rik antibody
- C76600 antibody
- Carnosinase , tissue antibody
see all
Images
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Western blot - Anti-CNDP2/CN2 antibody (ab241126)All lanes : Anti-CNDP2/CN2 antibody (ab241126) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 30 secondsLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-CNDP2/CN2 antibody (ab241126)CNDP2/CN2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241126 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241126 at 1 µg/ml.
Lane 1: ab241126 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared using NETN buffer.
Datasheets and documents
References
ab241126 has not yet been referenced specifically in any publications.
