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Anti-CNKSR3 antibody (ab234708)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-CNKSR3 antibody (ab234708)
  • Western blot - Anti-CNKSR3 antibody (ab234708)

Key features and details

  • Rabbit polyclonal to CNKSR3
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CNKSR3 antibody
    See all CNKSR3 primary antibodies
  • Description

    Rabbit polyclonal to CNKSR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human CNKSR3 aa 301-555.
    Sequence:

    PAPLKNLRWKPPLVQTSPPPATTQSPESTMDTSLKKEKSAILDLYIPPPP AVPYSPRDENGSFVYGGSSKCKQPLPGPKGSESPNSFLDQESRRRRFTIA DSDQLPGYSVETNILPTKMREKTPSYGKPRPLSMPADGNWMGIVDPFARP RGHGRKGEDALCRYFSNERIPPIIEESSSPPYRFSRPTTERHLVRGADYI RGSRCYINSDLHSSATIPFQEEGTKKKSGSSATKSSSTEPSLLVSWFTRL KLLTH


    Database link: Q6P9H4
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse spleen lysate. ICC/IF: HepG2 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab29293)
    • Mouse spleen tissue lysate - total protein (ab7937)

Applications

Our Abpromise guarantee covers the use of ab234708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ICC/IF 1/50 - 1/200.

Target

  • Relevance

    CNKSR3 belongs to the CNKSR family. It contains 1 CRIC domain, 1 DUF1170 domain, 1 PDZ (DHR) domain and 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Cell Membrane and Cytoplasmic
  • Database links

    • Entrez Gene: 154043 Human
    • Entrez Gene: 215748 Mouse
    • Entrez Gene: 308113 Rat
    • SwissProt: Q6P9H4 Human
    • SwissProt: Q8BMA3 Mouse
    • SwissProt: Q5SGD7 Rat
    • Alternative names

      • CNK homolog protein 3 antibody
      • CNK3 antibody
      • CNKSR family member 3 antibody
      • Connector enhancer of kinase suppressor of ras 3 antibody
      • Connector enhancer of KSR 3 antibody
      • MAGI1 antibody
      • Maguin like protein antibody
      • Membrane associated guanylate kinase interacting protein like 1 antibody
      • Membrane associated guanylate kinase, WW and PDZ domain containing 1 antibody
      • RP11 486M3 1 antibody
      • RP11 486M3.1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CNKSR3 antibody (ab234708)
      Immunocytochemistry/ Immunofluorescence - Anti-CNKSR3 antibody (ab234708)

      HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CNKSR3 (green) using ab234708 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

    • Western blot - Anti-CNKSR3 antibody (ab234708)
      Western blot - Anti-CNKSR3 antibody (ab234708)
      Anti-CNKSR3 antibody (ab234708) at 1/200 dilution + Mouse spleen lysate

      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution

      Predicted band size: 62 kDa
      Observed band size: 62 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab234708? Please let us know so that we can cite the reference in this datasheet.

    ab234708 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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