Overview

  • Product name
    Anti-CNNM1 antibody
  • Description
    Rabbit polyclonal to CNNM1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence:TACHMDSSPQSPDMEAFTDGDSTKAPTTRGTPQTPKDDPAI TLLNNRNSLPCSRSDGLRS PSEVVYLRMEELAFTQEEMTDFEEHSTQQLT, corresponding to amino acids 791-881 of Human CNNM1 Isoform 1 (Q9NRU3).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human testis tissue Human plasma tissue Human liver tissue lysate Human tonsil tissue lysate RT 4 cell line lysate U 251 MG cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab122648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
WB 1/250 - 1/500.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Probable metal transporter.
  • Tissue specificity
    Restricted to brain and testis.
  • Sequence similarities
    Belongs to the ACDP family.
    Contains 2 CBS domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • ACDP1 antibody
      • Ancient conserved domain protein 1 antibody
      • Ancient conserved domain-containing protein 1 antibody
      • CNNM1 antibody
      • CNNM1_HUMAN antibody
      • Cyclin-M1 antibody
      • FLJ31632 antibody
      • Metal transporter CNNM1 antibody
      • OTTHUMP00000020253 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CNNM1 antibody (ab122648)
      Immunocytochemistry/ Immunofluorescence - Anti-CNNM1 antibody (ab122648)
      Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122648 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Western blot - Anti-CNNM1 antibody (ab122648)
      Western blot - Anti-CNNM1 antibody (ab122648)
      All lanes : Anti-CNNM1 antibody (ab122648) at 1/250 dilution

      Lane 1 : RT 4 cell line lysate
      Lane 2 : U 251 MG cell line lysate
      Lane 3 : Human plasma
      Lane 4 : Human liver tissue lysate
      Lane 5 : Human tonsil tissue lysate
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNNM1 antibody (ab122648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNNM1 antibody (ab122648)
      ab122648, at 1/1000, staining CNNM1 in paraffin embedded Human testis by Immunohistochemistry.

    References

    ab122648 has not yet been referenced specifically in any publications.

    Publishing research using ab122648? Please let us know so that we can cite the reference in this datasheet.

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