Anti-CNNM1 antibody (ab122648)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CNNM1 antibody -
Description
Rabbit polyclonal to CNNM1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence:TACHMDSSPQSPDMEAFTDGDSTKAPTTRGTPQTPKDDPAI TLLNNRNSLPCSRSDGLRS PSEVVYLRMEELAFTQEEMTDFEEHSTQQLT, corresponding to amino acids 791-881 of Human CNNM1 Isoform 1 (Q9NRU3).
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Positive control
- Human testis tissue Human plasma tissue Human liver tissue lysate Human tonsil tissue lysate RT 4 cell line lysate U 251 MG cell line lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| WB | 1/250 - 1/500. | |
| IHC-P | 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Probable metal transporter. -
Tissue specificity
Restricted to brain and testis. -
Sequence similarities
Belongs to the ACDP family.
Contains 2 CBS domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 26507 Human
- Omim: 607802 Human
- SwissProt: Q9NRU3 Human
- Unigene: 274579 Human
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Alternative names
- ACDP1 antibody
- Ancient conserved domain protein 1 antibody
- Ancient conserved domain-containing protein 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CNNM1 antibody (ab122648)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122648 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-CNNM1 antibody (ab122648)All lanes : Anti-CNNM1 antibody (ab122648) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNNM1 antibody (ab122648)ab122648, at 1/1000, staining CNNM1 in paraffin embedded Human testis by Immunohistochemistry.
Protocols
Datasheets and documents
References
ab122648 has not yet been referenced specifically in any publications.
