Anti-CNOT3 antibody (ab154276)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CNOT3 antibody -
Description
Rabbit polyclonal to CNOT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit
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Immunogen
Recombinant fragment corresponding to Human CNOT3 aa 522-753 (C terminal).
Database link: O75175 -
Positive control
- IMR32 whole cell lysate; NT2D1, Jurkat and HeLa cell lines; Human H441 xenograft tissue; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 82 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
The CCR4-NOT complex functions as general transcription regulation complex. -
Tissue specificity
Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. -
Sequence similarities
Belongs to the CNOT2/3/5 family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4849 Human
- Entrez Gene: 232791 Mouse
- Entrez Gene: 308311 Rat
- Omim: 604910 Human
- SwissProt: O75175 Human
- SwissProt: Q8K0V4 Mouse
- Unigene: 343571 Human
- Unigene: 358731 Mouse
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Alternative names
- CCR4 associated factor 3 antibody
- CCR4-associated factor 3 antibody
- CCR4-NOT transcription complex subunit 3 antibody
see all
Images
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Western blot - Anti-CNOT3 antibody (ab154276)Anti-CNOT3 antibody (ab154276) at 1/3000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CNOT3 antibody (ab154276)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/200 dilution. Lower panel: Costained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT3 antibody (ab154276)Immunohistochemical analysis of paraffin-embedded Human H441 xenograft, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/100 dilution.
Protocols
References
ab154276 has not yet been referenced specifically in any publications.
