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Rabbit polyclonal to CNOT3
HumanPredicted to work with:
Mouse, Rat, Rabbit
Recombinant fragment corresponding to Human CNOT3 aa 522-753 (C terminal).
Database link: O75175
- IMR32 whole cell lysate; NT2D1, Jurkat and HeLa cell lines; Human H441 xenograft tissue; HeLa cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 82 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/100 - 1/1000.
The CCR4-NOT complex functions as general transcription regulation complex.
Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Belongs to the CNOT2/3/5 family.
Information by UniProt
- CCR4 associated factor 3 antibody
- CCR4-associated factor 3 antibody
- CCR4-NOT transcription complex subunit 3 antibody
Western blot - Anti-CNOT3 antibody (ab154276)
Anti-CNOT3 antibody (ab154276) at 1/3000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-CNOT3 antibody (ab154276)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/200 dilution. Lower panel: Costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT3 antibody (ab154276)
Immunohistochemical analysis of paraffin-embedded Human H441 xenograft, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"