Anti-CNOT3 antibody (ab154276)
Key features and details
- Rabbit polyclonal to CNOT3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CNOT3 antibody -
Description
Rabbit polyclonal to CNOT3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Recombinant fragment corresponding to Human CNOT3 aa 522-753 (C terminal).
Database link: O75175 -
Positive control
- IMR32 whole cell lysate; NT2D1, Jurkat and HeLa cell lines; Human H441 xenograft tissue; HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Rabbit
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 82 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 82 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
Target
-
Function
The CCR4-NOT complex functions as general transcription regulation complex. -
Tissue specificity
Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. -
Sequence similarities
Belongs to the CNOT2/3/5 family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4849 Human
- Entrez Gene: 232791 Mouse
- Entrez Gene: 308311 Rat
- Omim: 604910 Human
- SwissProt: O75175 Human
- SwissProt: Q8K0V4 Mouse
- Unigene: 343571 Human
- Unigene: 358731 Mouse
-
Alternative names
- CCR4 associated factor 3 antibody
- CCR4-associated factor 3 antibody
- CCR4-NOT transcription complex subunit 3 antibody
see all
Images
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Western blot - Anti-CNOT3 antibody (ab154276)Anti-CNOT3 antibody (ab154276) at 1/3000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CNOT3 antibody (ab154276)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/200 dilution. Lower panel: Costained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT3 antibody (ab154276)Immunohistochemical analysis of paraffin-embedded Human H441 xenograft, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/100 dilution.
Protocols
References (1)
ab154276 has been referenced in 1 publication.
- Park J et al. UPF1/SMG7-dependent microRNA-mediated gene regulation. Nat Commun 10:4181 (2019). PubMed: 31519907
