Anti-CNOT4 antibody (ab214937)

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Overview

  • Product name

    Anti-CNOT4 antibody

  • Description

    Rabbit polyclonal to CNOT4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Human CNOT4 aa 331-476.
    Sequence:

    TESQSLFSDNFRHPNPIPSGLPPFPSSPQTSSDWPTAPEPQSLFTSETIP VSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKELSVQDQPS LSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRF


    Database link: O95628

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • NIH 3T3, NBT II, RT 4 and U 251MG cell lysates; Human liver and tonsil tissue lysates; Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab214937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 63 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 C3H1-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.

  • Post-translational
    modifications

    Autoubiquitinated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CCR4 NOT transcription complex subunit 4 antibody
    • CCR4-associated factor 4 antibody
    • CCR4-NOT transcription complex subunit 4 antibody
    • CLONE243 antibody
    • CNOT 4 antibody
    • Cnot4 antibody
    • CNOT4_HUMAN antibody
    • E3 ubiquitin-protein ligase CNOT4 antibody
    • NOT 4 antibody
    • NOT4 (negative regulator of transcription 4 yeast) homolog antibody
    • NOT4 antibody
    • NOT4H antibody
    • Potential transcriptional repressor NOT4Hp antibody
    see all

Images

  • Western blot - Anti-CNOT4 antibody (ab214937)
    Western blot - Anti-CNOT4 antibody (ab214937)
    All lanes : Anti-CNOT4 antibody (ab214937) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251MG cell lysate
    Lane 3 : Human plasma (IgG/HSA depleted) lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Predicted band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT4 antibody (ab214937)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT4 antibody (ab214937)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling CNOT4 with ab214937 at 1/50 dilution.

  • Western blot - Anti-CNOT4 antibody (ab214937)
    Western blot - Anti-CNOT4 antibody (ab214937)
    All lanes : Anti-CNOT4 antibody (ab214937) at 1/250 dilution

    Lane 1 : NIH 3T3 cell lysate
    Lane 2 : NBT II cell lysate

    Predicted band size: 63 kDa

References

ab214937 has not yet been referenced specifically in any publications.

Publishing research using ab214937? Please let us know so that we can cite the reference in this datasheet.

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