Recombinant Anti-CNOT6 antibody [EPR22022] (ab221151)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22022] to CNOT6
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CNOT6 antibody [EPR22022]
See all CNOT6 primary antibodies -
Description
Rabbit monoclonal [EPR22022] to CNOT6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat and MCF7 whole cell lysates. IP: Jurkat and HeLa whole cell lysates. Human testis, ovaries and heart tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22022 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Predicted molecular weight: 63 kDa.
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IP |
1/30.
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Notes |
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WB
1/1000. Predicted molecular weight: 63 kDa. |
IP
1/30. |
Target
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Function
Poly(A) nuclease involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Has 3'-5' RNase activity. The CCR4-NOT complex functions as general transcription regulation complex. -
Sequence similarities
Belongs to the CCR4/nocturin family.
Contains 4 LRR (leucine-rich) repeats. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57472 Human
- Omim: 608951 Human
- SwissProt: Q9ULM6 Human
- Unigene: 608380 Human
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Alternative names
- A230103N10Rik antibody
- AA407540 antibody
- AW456442 antibody
see all
Images
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All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution
Lane 1 : Human testis lysate with 5% NFDM/TBST
Lane 2 : Human ovary lysate with 5% NFDM/TBST
Lane 3 : Human heart lysate with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsThe expression profile is consistent with what has been described in the literature (PMID: 21741365).
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All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution
Lane 1 : His-tagged human CNOT6 recombinant protein(aa1-210) with 5% NFDM/TBST
Lane 2 : His-tagged human CNOT6L recombinant protein(aa1-210) with 5% NFDM/TBST
Lysates/proteins at 0.002 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 63 kDa
Exposure time: 3 seconds -
All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa whole cell lysate with CNOT6 Immunogen peptide
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 63 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The reactivity of the antibody with the target protein in the HeLa lysate was blocked by the inclusion of the peptide antigen.
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All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Human ovary cancer lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 63 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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CNOT6 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab221151 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221151 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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CNOT6 was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab221151 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221151 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab221151 has been referenced in 1 publication.
- Liu J et al. YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways. Cell Rep 32:108120 (2020). PubMed: 32905781