Overview

  • Product name

    Anti-CNOT6 antibody [EPR22022]
    See all CNOT6 primary antibodies
  • Description

    Rabbit monoclonal [EPR22022] to CNOT6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CNOT6 aa 1-200. The exact sequence is proprietary.
    Database link: Q9ULM6

  • Positive control

    • WB: HeLa, Jurkat and MCF7 whole cell lysates. IP: Jurkat and HeLa whole cell lysates. Human testis, ovaries and heart tissue lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22022
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 63 kDa.
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Poly(A) nuclease involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Has 3'-5' RNase activity. The CCR4-NOT complex functions as general transcription regulation complex.
    • Sequence similarities

      Belongs to the CCR4/nocturin family.
      Contains 4 LRR (leucine-rich) repeats.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • A230103N10Rik antibody
      • AA407540 antibody
      • AW456442 antibody
      • carbon catabolite repression 4 protein antibody
      • Carbon catabolite repressor protein 4 homolog antibody
      • CCR4 antibody
      • CCR4 carbon catabolite repression 4 like antibody
      • CCR4 carbon catabolite repression 4-like antibody
      • CCR4-NOT transcription complex subunit 6 antibody
      • CCR4-NOT transcription complex, subunit 6 antibody
      • Cnot6 antibody
      • CNOT6_HUMAN antibody
      • Cytoplasmic deadenylase antibody
      • EC 3.1.-.- antibody
      • fa03c11 antibody
      • fc17f01 antibody
      • KIAA1194 antibody
      • MGC98472 antibody
      • OTTHUMP00000161544 antibody
      • wu:fa03c11 antibody
      • wu:fc17f01 antibody
      • zgc:65822 antibody
      see all

    Images

    • All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

      Lane 1 : Human testis lysate with 5% NFDM/TBST
      Lane 2 : Human ovary lysate with 5% NFDM/TBST
      Lane 3 : Human heart lysate with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 63 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?


      Exposure time: 37 seconds


      The expression profile is consistent with what has been described in the literature (PMID: 21741365).

    • All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

      Lane 1 : His-tagged human CNOT6 recombinant protein(aa1-210) with 5% NFDM/TBST
      Lane 2 : His-tagged human CNOT6L recombinant protein(aa1-210) with 5% NFDM/TBST

      Lysates/proteins at 0.002 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 63 kDa


      Exposure time: 3 seconds
    • All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HeLa whole cell lysate with CNOT6 Immunogen peptide

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 63 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The reactivity of the antibody with the target protein in the HeLa lysate was blocked by the inclusion of the peptide antigen.

    • All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 4 : Human ovary cancer lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 63 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • CNOT6 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: HeLa whole cell lysate 10 µg (Input). 

      Lane 2: ab221151 IP in HeLa whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221151 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    • CNOT6 was immunoprecipitated from 0.35 mg of Jurkat (human  T cell leukemia cell line from peripheral blood) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

      Lane 1: Jurkat whole cell lysate 10 µg (Input). 

      Lane 2: ab221151 IP in Jurkat whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221151 in Jurkat whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    References

    ab221151 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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