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  1. Link

    cnpase-antibody-11-5b-ab6319.pdf

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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker
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Validated using a knockout cell line

Anti-CNPase antibody [11-5B] (ab6319)

  • Datasheet
Reviews (38)Q&A (7)References (103)

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Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [11-5B] (ab6319)
  • Western blot - Anti-CNPase antibody [11-5B] (ab6319)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] (ab6319)
  • Western blot - Anti-CNPase antibody [11-5B] (ab6319)
  • Western blot - Anti-CNPase antibody [11-5B] (ab6319)
  • Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [11-5B] (ab6319)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] (ab6319)
  • Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [11-5B] (ab6319)
  • Western blot - Anti-CNPase antibody [11-5B] (ab6319)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] (ab6319)

Key features and details

  • Mouse monoclonal [11-5B] to CNPase
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 HRP

You may also be interested in

Primary
Product image
Alexa Fluor® 647 Anti-CNPase antibody [11-5B] (ab201679)
Primary
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Anti-Tau (phospho S396) antibody [E178] - BSA and Azide free (ab218600)
Knockout
Product image
Human CNP (CNPase) knockout HeLa cell line (ab264949)

View more associated products

Overview

  • Product name

    Anti-CNPase antibody [11-5B]
    See all CNPase primary antibodies
  • Description

    Mouse monoclonal [11-5B] to CNPase
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Cow, Dog, Pig, Rhesus monkeyDoes not react with: Chicken, Guinea pig
  • Immunogen

    Full length native protein (purified) corresponding to Human CNPase.

  • Positive control

    • WB: HeLa and Hap1 cell lysates; Human and Mouse Spinal Cord and Brain tissue lysates; Rat Brain tissue lysate. IHC-P: FFPE human cerebral cortex tissue sections. ICC: Primary hippocampal rat neurons/glia, DIV14. cells
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    11-5B
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Associated products

  • Alternative Versions

    • HRP Anti-CNPase antibody [11-5B] (ab201678)
    • Alexa Fluor® 647 Anti-CNPase antibody [11-5B] (ab201679)
    • Anti-CNPase antibody [11-5B] - BSA and Azide free (ab237961)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • KO cell lines

    • Human CNP (CNPase) knockout HeLa cell line (ab264949)
  • KO cell lysates

    • Human CNP (CNPase) knockout HeLa cell lysate (ab256877)
  • KO cell pellets

    • Human CNP (CNPase) knockout HeLa cell pellet (ab278945)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (15)
Use at an assay dependent concentration.
WB (8)
Use a concentration of 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ICC/IF (7)
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ICC/IF
Use at an assay dependent concentration.

Target

  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family.
  • Cellular localization

    Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P09543 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 280752 Cow
    • Entrez Gene: 607694 Dog
    • Entrez Gene: 1267 Human
    • Entrez Gene: 12799 Mouse
    • Entrez Gene: 493772 Pig
    • Entrez Gene: 25275 Rat
    • Entrez Gene: 641337 Rhesus monkey
    • Omim: 123830 Human
    • SwissProt: P06623 Cow
    • SwissProt: P09543 Human
    • SwissProt: P16330 Mouse
    • SwissProt: P18104 Pig
    • SwissProt: P13233 Rat
    • SwissProt: P56283 Sheep
    • Unigene: 273621 Human
    • Unigene: 15711 Mouse
    • Unigene: 31762 Rat
    see all
  • Alternative names

    • 2'' antibody
    • 2'3' cyclic nucleotide 3' phosphodiesterase antibody
    • 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
    • CN37_HUMAN antibody
    • CNP 1 antibody
    • CNP antibody
    • CNP1 antibody
    • CNPase antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [11-5B] (ab6319)
    Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [11-5B] (ab6319)

    ab6319 staining CNPase in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6319 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Western blot - Anti-CNPase antibody [11-5B] (ab6319)
    Western blot - Anti-CNPase antibody [11-5B] (ab6319)
    All lanes : Anti-CNPase antibody [11-5B] (ab6319) at 5 µg/ml

    Lane 1 : Wild-type HAP1 cell lysate at 40 µg
    Lane 2 : CNPase knockout HAP1 cell lysate at 40 µg
    Lane 3 : Human brain whole cell lysate at 20 µg

    Predicted band size: 48 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab6319 observed at 48 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab6319 was shown to recognize CNPase in wild-type HAP1 cells as signal was lost at the expected MW in CNPase knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CNPase knockout samples were subjected to SDS-PAGE. Ab6319 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 5 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] (ab6319)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] (ab6319)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    IHC-P image of CNPase staining on rat brain sections using ab6319 (1/1600). heat mediated antigen retrieval on paraffin embedded sections was performed using citric acid. The sections were then blocked with 1% BSA for 10 min at 21°C. The primary antibody was incubated for 16 hours at 21°C. The sections were then incubated in Goat anti-mouse (Biotin) at 1:200.

    See Abreview

  • Western blot - Anti-CNPase antibody [11-5B] (ab6319)
    Western blot - Anti-CNPase antibody [11-5B] (ab6319)
    All lanes : Anti-CNPase antibody [11-5B] (ab6319)

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CNP knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab6319 observed at 48 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.

     ab6319 was shown to react with CNPase in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264949 (knockout cell lysate ab256877) was used. Wild-type HeLa and CNP knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab6319 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 5 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CNPase antibody [11-5B] (ab6319)
    Western blot - Anti-CNPase antibody [11-5B] (ab6319)
    All lanes : Anti-CNPase antibody [11-5B] (ab6319) at 1/100 dilution

    Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)
    Lane 3 : Spinal Cord (Mouse) Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate
    Lane 5 : Spinal Cord (Rat) Tissue Lysate
    Lane 6 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa


    Exposure time: 1 minute


    This antibody was raised against full length native CNPase and is predicted to recognize both isoforms. The predicted molecular weights of isoforms CNPI and CNPII are 45- and 48-kDa respectively.
  • Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [11-5B] (ab6319)
    Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [11-5B] (ab6319)This image is courtesy of an anonymous abreview.
    ab6319 staining CNPase in rat oligodendrocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, blocked using 5% serum for 10 minutes at 25°C, then incubated with ab6319 at a 1/200 dilution for 2 hours at 25°C. The secondary used was a goat anti-mouse Cy3 conjugated polyclonal at a 1/100 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] (ab6319)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] (ab6319)

    IHC image of CNPase staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6319, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [11-5B] (ab6319)
    Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [11-5B] (ab6319)This image is courtesy of an anonymous Abreview

    ab6319 staining CNPase in the rat oligodendrocytes by ICC/IF (Immunoytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 5% BSA for 1 hour at 37°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-mouse IgG polyclonal (1:200) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-CNPase antibody [11-5B] (ab6319)
    Western blot - Anti-CNPase antibody [11-5B] (ab6319)This image is courtesy of an anonymous Abreview
    All lanes : Anti-CNPase antibody [11-5B] (ab6319) at 1/750 dilution

    All lanes : Spinal Cord homogenate (whole tissue lysate)

    Lysates/proteins at 2 µg per lane.

    Secondary
    All lanes : HRP conjugated sheep anti-mouse IgG

    Predicted band size: 48 kDa
    Observed band size: 45,47 kDa why is the actual band size different from the predicted?

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] (ab6319)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] (ab6319)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    ab6319 staining CNPase in Dog Cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1500 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouset IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (103)

Publishing research using ab6319? Please let us know so that we can cite the reference in this datasheet.

ab6319 has been referenced in 103 publications.

  • Brooks LJ  et al. The white matter is a pro-differentiative niche for glioblastoma. Nat Commun 12:2184 (2021). PubMed: 33846316
  • Ping S  et al. Brain-derived CCR5 Contributes to Neuroprotection and Brain Repair after Experimental Stroke. Aging Dis 12:72-92 (2021). PubMed: 33532129
  • Jia J  et al. Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1. Stem Cell Res Ther 12:169 (2021). PubMed: 33691791
  • Kambe Y  et al. The pivotal role of pituitary adenylate cyclase-activating polypeptide for lactate production and secretion in astrocytes during fear memory. Pharmacol Rep N/A:N/A (2021). PubMed: 33835466
  • Aoto K  et al. ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H+-ATPases is essential for brain development in humans and mice. Nat Commun 12:2107 (2021). PubMed: 33833240
View all Publications for this product

Customer reviews and Q&As

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1-10 of 38 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CNPase antibody [11-5B]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6
Permeabilization
No
Specification
Brain
Blocking step
Hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 16 2023

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CNPase antibody [11-5B]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 30 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CNPase antibody [11-5B]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 30 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CNPase antibody [11-5B]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Zebrafish Tissue sections (Embryo)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Embryo
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 24 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-CNPase antibody [11-5B]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain cell culture)
Permeabilization
Yes - 0.05% Tween 20
Specification
Brain cell culture
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 18 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CNPase antibody [11-5B]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Brain
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 11 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CNPase antibody [11-5B]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Brain
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 11 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-CNPase antibody [11-5B]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain)
Permeabilization
Yes - 0.1% Triton X100
Specification
Brain
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 31 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CNPase antibody [11-5B]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Brain
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. David Ivancic

Verified customer

Submitted Jun 25 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CNPase antibody [11-5B]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph 9
Permeabilization
No
Specification
brain tissue
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Formaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Markus Kipp

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Submitted Sep 07 2018

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