Key features and details
- Mouse monoclonal [1H10] to CNPase
- Suitable for: IHC, WB
- Reacts with: Mouse, Rat
- Isotype: IgG1
Product nameAnti-CNPase antibody [1H10]
See all CNPase primary antibodies
DescriptionMouse monoclonal [1H10] to CNPase
Tested applicationsSuitable for: IHC, WBmore details
Species reactivityReacts with: Mouse, Rat
Recombinant full length protein corresponding to Human CNPase.
Database link: P09543
- WB: Rat brain and spinal cord mouse brain and spinal cord. IHC: Rat cerebellum (oligodendrocytes).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from Tissue Culture supernatant.
Our Abpromise guarantee covers the use of ab277621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Perfusion-fixed with 4% paraformaldehyde for 24 hours, free floating sections.
|WB||1/1000 - 1/2000. Detects a band of approximately 48 kDa.|
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
Cellular localizationMembrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
Perfusion-fixed, free floating sections of rat cerebellum tissue stained for CNPase using ab277621 (green) at 1/500 dilution, and counterstained with chicken pAb to NF-M (red) at 1/1000 dilution in immunohistochemical analysis.
Lanes 2-5 : Anti-CNPase antibody [1H10] (ab277621) at 1/2000 dilution
Lane 1 : Molecular Weight ladder
Lane 2 : rat brain
Lane 3 : rat spinal cord
Lane 4 : mouse brain
Lane 5 : mouse spinal cord
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277621 has not yet been referenced specifically in any publications.