Anti-CNPase antibody (ab139744)
Key features and details
- Chicken polyclonal to CNPase
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgY
Overview
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Product name
Anti-CNPase antibody
See all CNPase primary antibodies -
Description
Chicken polyclonal to CNPase -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CNPase aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab139999) -
Positive control
- This antibody gave a positive signal in the following lysates: mouse spinal cord; mouse brain; rat brain.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab139744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
Target
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Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. -
Cellular localization
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 12799 Mouse
- Entrez Gene: 25275 Rat
- SwissProt: P16330 Mouse
- SwissProt: P13233 Rat
- Unigene: 15711 Mouse
- Unigene: 31762 Rat
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Alternative names
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
see all
Images
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Western blot - Anti-CNPase antibody (ab139744)All lanes : Anti-CNPase antibody (ab139744) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 2 : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution (.)
Lanes 3-4 : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 45 kDa (possible isoform)
Exposure time: 90 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139744 overnight at 4°C. Antibody binding was detected using an anti-chicken antibody conjugated to HRP, and visualised using ECL development solution. The bands observed at 45- and 48-kDa correspond to the predicted molecular weights of isoforms CNPI and CNPII..
Datasheets and documents
References (0)
ab139744 has not yet been referenced specifically in any publications.
