Anti-CNPase antibody (ab139744)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CNPase antibody
See all CNPase primary antibodies -
Description
Chicken polyclonal to CNPase -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CNPase aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab139999) -
Positive control
- This antibody gave a positive signal in the following lysates: mouse spinal cord; mouse brain; rat brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab139744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
Target
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Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. -
Cellular localization
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 12799 Mouse
- Entrez Gene: 25275 Rat
- SwissProt: P16330 Mouse
- SwissProt: P13233 Rat
- Unigene: 15711 Mouse
- Unigene: 31762 Rat
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Alternative names
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
see all
Images
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Western blot - Anti-CNPase antibody (ab139744)All lanes : Anti-CNPase antibody (ab139744) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 2 : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution (.)
Lanes 3-4 : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 45 kDa (possible isoform)
Exposure time: 90 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139744 overnight at 4°C. Antibody binding was detected using an anti-chicken antibody conjugated to HRP, and visualised using ECL development solution. The bands observed at 45- and 48-kDa correspond to the predicted molecular weights of isoforms CNPI and CNPII..
Protocols
Datasheets and documents
References
ab139744 has not yet been referenced specifically in any publications.
