Observed band sizes: 46 and 48kDa Expected band sizes: 46 and 48kDa
ab18527 specifically recognises both isoforms of Human CNPase. The activity of the antibody is blocked by the addition of the peptide ab19423.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody (ab18527)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom
IHC-P image of CNPase staining on human brain section using ab18527 (1:2000). The paraffin embedded section was subjected to heat mediated antigen retrieval using citric acid. The sections were then blocked using 1% BSA at 21°C for 10 min. ab18527 was incubated at 21°C for 16 hours. The secondary antibody was goat polyclonal anti-rabbit IgG conjugated to biotin (1:200)