Anti-CNPase antibody (ab227218)
Key features and details
- Rabbit polyclonal to CNPase
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CNPase antibody
See all CNPase primary antibodies -
Description
Rabbit polyclonal to CNPase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human CNPase (internal sequence). The exact sequence is proprietary.
Database link: P09543 -
Positive control
- WB: HeLa cell extract; MOLT-4 whole cell lysate; Mouse and rat brain tissue extracts. IHC-P: U87 xenograft tissue. IHC-Fr: Adult mouse cerebellum and hippocampus tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/10000. Predicted molecular weight: 45,48 kDa.
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IHC-Fr |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/10000. Predicted molecular weight: 45,48 kDa. |
IHC-Fr
1/100 - 1/1000. |
Target
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Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. -
Cellular localization
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 1267 Human
- Entrez Gene: 12799 Mouse
- Entrez Gene: 25275 Rat
- Entrez Gene: 641337 Rhesus monkey
- Omim: 123830 Human
- SwissProt: P09543 Human
- SwissProt: P16330 Mouse
- SwissProt: P13233 Rat
see all -
Alternative names
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
see all
Images
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All lanes : Anti-CNPase antibody (ab227218) at 1/5000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 45,48 kDa10% SDS-PAGE gel.
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Anti-CNPase antibody (ab227218) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 45,48 kDa10% SDS-PAGE gel.
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All lanes : Anti-CNPase antibody (ab227218) at 1/500 dilution
Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : CNPase knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 45,48 kDa10% SDS-PAGE gel.
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Paraffin-embedded U87 (human) xenograft tissue stained for CNPase using ab227218 at 1/500 dilution in immunohistochemical analysis.
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Frozen sections of adult mouse cerebellum tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/500 dilution (red). Nuclear Counterstain: Fluoroshield with DAPI (blue).
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Frozen sections of adult mouse hippocampus tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain: NeuN (red), stained by NeuN antibody at 1/500 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab227218 has been referenced in 3 publications.
- Shen W et al. Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury. Dis Model Mech 16:N/A (2023). PubMed: 36518009
- Dion V et al. Cyclin-dependent kinase 7 contributes to myelin maintenance in the adult central nervous system and promotes myelin gene expression. Glia 70:1652-1665 (2022). PubMed: 35488490
- Fan H et al. MsrA Suppresses Inflammatory Activation of Microglia and Oxidative Stress to Prevent Demyelination via Inhibition of the NOX2-MAPKs/NF-?B Signaling Pathway. Drug Des Devel Ther 14:1377-1389 (2020). PubMed: 32308370