Overview

  • Product name

  • Description

    Rabbit polyclonal to CNPase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human CNPase (internal sequence). The exact sequence is proprietary.
    Database link: P09543

  • Positive control

    • WB: HeLa cell extract; MOLT-4 whole cell lysate; Mouse and rat brain tissue extracts. IHC-P: U87 xenograft tissue. IHC-Fr: Adult mouse cerebellum and hippocampus tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab227218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/10000. Predicted molecular weight: 45,48 kDa.
IHC-Fr 1/100 - 1/1000.

Target

Images

  • Anti-CNPase antibody (ab227218) at 1/500 dilution + CNPase Knockout HeLa cell extracts at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 45,48 kDa

  • Frozen sections of adult mouse cerebellum tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/500 dilution (red). Nuclear Counterstain: Fluoroshield with DAPI (blue).

  • All lanes : Anti-CNPase antibody (ab227218) at 1/500 dilution

    Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : CNPase knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 45,48 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded U87 (human) xenograft tissue stained for CNPase using ab227218 at 1/500 dilution in immunohistochemical analysis.

  • Frozen sections of adult mouse hippocampus tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain: NeuN (red), stained by NeuN antibody at 1/500 dilution. 

  • Anti-CNPase antibody (ab227218) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg

    Predicted band size: 45,48 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-CNPase antibody (ab227218) at 1/5000 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 45,48 kDa



    10% SDS-PAGE gel.

References

ab227218 has not yet been referenced specifically in any publications.

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