Anti-CNTF antibody (ab46172)
Key features and details
- Rabbit polyclonal to CNTF
- Suitable for: ICC/IF, ELISA, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CNTF antibody
See all CNTF primary antibodies -
Description
Rabbit polyclonal to CNTF -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant human CNTF.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000.
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/1000. |
ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. -
Tissue specificity
Nervous system. -
Sequence similarities
Belongs to the CNTF family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1270 Human
- Entrez Gene: 12803 Mouse
- Entrez Gene: 25707 Rat
- Omim: 118945 Human
- SwissProt: P26441 Human
- SwissProt: P51642 Mouse
- SwissProt: P20294 Rat
- Unigene: 524920 Human
see all -
Alternative names
- Ciliary neurotrophic factor antibody
- CNTF antibody
- CNTF_HUMAN antibody
- HCNTF antibody
Images
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ICC/IF image of ab46172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46172, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab46172 has been referenced in 9 publications.
- Wei W et al. Neurotrophic Treatment Initiated During Early Postnatal Development Prevents the Alzheimer-Like Behavior and Synaptic Dysfunction. J Alzheimers Dis 82:631-646 (2021). PubMed: 34057082
- Cheng XQ et al. Protein microarray analysis of cytokine expression changes in distal stumps after sciatic nerve transection. Neural Regen Res 15:503-511 (2020). PubMed: 31571662
- Si ZP et al. CNTF and Nrf2 Are Coordinately Involved in Regulating Self-Renewal and Differentiation of Neural Stem Cell during Embryonic Development. iScience 19:303-315 (2019). PubMed: 31404831
- Fan L et al. Polyaniline promotes peripheral nerve regeneration by enhancement of the brain-derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling pathway. Mol Med Rep 16:7534-7540 (2017). PubMed: 28944860
- Fan K et al. Hypomethylation of CNTFRa is associated with proliferation and poor prognosis in lower grade gliomas. Sci Rep 7:7079 (2017). PubMed: 28765641
- Huang L et al. Congenital absence of corticospinal tract does not severely affect plastic changes of the developing postnatal spinal cord. Neuroscience 301:338-50 (2015). IHC-P, WB ; Mouse . PubMed: 26079333
- Joly S et al. New mouse retinal stroke model reveals direction-selective circuit damage linked to permanent optokinetic response loss. Invest Ophthalmol Vis Sci 55:4476-89 (2014). PubMed: 24970264
- Severi I et al. Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus. Front Neurosci 7:263 (2013). PubMed: 24409114
- Paintlia MK et al. S-nitrosoglutathione induces ciliary neurotrophic factor expression in astrocytes, which has implications to protect the central nervous system under pathological conditions. J Biol Chem 288:3831-43 (2013). ICC/IF ; Rat . PubMed: 23264628