Overview

  • Product name

  • Description

    Rabbit polyclonal to CNTF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, Neutralising, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Highly pure (>98%) recombinant rCNTF (Rat Ciliary Neurotrophic Factor)

  • Positive control

    • Recombinant human CNTF protein (ab9786) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant rCNTF.
Neutralising Use a concentration of 0.002 - 0.005 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of rCNTF (0.15 ng/ml).
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function

    CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
  • Tissue specificity

    Nervous system.
  • Sequence similarities

    Belongs to the CNTF family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ciliary neurotrophic factor antibody
    • CNTF antibody
    • CNTF_HUMAN antibody
    • HCNTF antibody

Images

  • Immunohistochemical analysis of PFA perfusion fixed frozen rat brain, staining CNTF (green) with ab9785 at 0.25 µg/ml. A peroxidase conjugated secondary antibody was added and staining was detected using a fluorescein Tyramide Signal Amplification (TSA™) reagent.

References

This product has been referenced in:

  • Zacks DN  et al. Activation of signaling pathways and stress-response genes in an experimental model of retinal detachment. Invest Ophthalmol Vis Sci 47:1691-5 (2006). WB ; Rat . Read more (PubMed: 16565410) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

Indeed, I can confirm that PBS as dilution buffer is suitable for all three antibodies and the protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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