Key features and details
- Mouse monoclonal [AS23] to CNTF
- Suitable for: ELISA, WB, IP
- Reacts with: Human
- Isotype: IgG1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab17284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: This antibody has been used as a coating antibody at 10 ug/ml in a two site ELISA with PS2-B. Typically the ELISA has a working range of 0-20000 pg/ml and a sensitivity of 10 pg/ml when used with biotin conjugated CNTF antibody. Recommended sample volumes are 25-200 µl. Recovery of hCNTF in human plasma is typically > 85 in the ELISA.
IP: This antibody has been used to immunoprecipitate >90% hCNTF in solution using a double-antibody method.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Tissue specificityNervous system.
Sequence similaritiesBelongs to the CNTF family.
- Information by UniProt
- Ciliary neurotrophic factor antibody
- CNTF antibody
- CNTF_HUMAN antibody
- HCNTF antibody
ab17284 has been referenced in 1 publication.
- Millán-Rivero JE et al. Human Wharton's jelly mesenchymal stem cells protect axotomized rat retinal ganglion cells via secretion of anti-inflammatory and neurotrophic factors. Sci Rep 8:16299 (2018). PubMed: 30389962